Seroatlas · Human Serome Atlas

CARD16

Caspase recruitment domain-containing protein 16

Also known as: CAR16_HUMAN, COP, COP1, LLID-114769, PSEUDO-ICE

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5EG05
Gene: CARD16
Ensembl: ENSG00000204397
Chromosome: 11
Canonical length: 197 aa
Protein class: Predicted intracellular proteins
Subcellular location: Mitochondria
Quaternary structure: Homooligomer

OverviewNCBI Gene

Enables several functions, including CARD domain binding activity; cysteine-type endopeptidase inhibitor activity; and enzyme binding activity. Involved in cellular response to lipopolysaccharide; regulation of gene expression; and regulation of signal transduction. Part of protease inhibitor complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

197 residues, UniProt reviewed canonical sequence.

>Q5EG05|CARD16
     1  MADKVLKEKR KLFIHSMGEG TINGLLDELL QTRVLNQEEM EKVKRENATV MDKTRALIDS
    61  VIPKGAQACQ ICITYICEED SYLAETLGLS AALQAVQDNP AMPTCSSPEG RIKLCFLEDA
   121  QRIWKQKLQR CHVQNTIIKW SERYTSGSFE MQWLFLRTNF IERFWRNILL LPLHKGSLYP
   181  RIPGLGKELQ TGTHKLS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CARD16 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.46
Highest tissue expression: 71 nTPM

Expression across tissuesHPA

Tissue

spleen: 71 nTPM
placenta: 59 nTPM
lung: 53 nTPM
bone marrow: 48 nTPM
small intestine: 42 nTPM
rectum: 42 nTPM

Single-cell type

neutrophils: 323 nCPM
cdc: 207 nCPM
monocytes: 187 nCPM
kupffer cells: 182 nCPM
platelets: 134 nCPM
hepatic stellate cells: 103 nCPM

Immune cell

neutrophil: 1,328 nTPM
intermediate monocyte: 1,227 nTPM
non-classical monocyte: 1,136 nTPM
total PBMC: 955 nTPM
classical monocyte: 933 nTPM
eosinophil: 695 nTPM

Brain region

cerebral cortex: 9 nTPM
choroid plexus: 5.1 nTPM
white matter: 5 nTPM
pons: 4.7 nTPM
medulla oblongata: 4.6 nTPM
midbrain: 3.7 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.97
gnomAD pLI: 0.38
gnomAD missense Z: -0.47
DepMap mean gene effect: -0.07
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CARD16 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CARD16 as an antibody target. Whether an autoantibody or antibody against CARD16 could matter depends on whether native CARD16 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CARD16 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CARD16 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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