Seroatlas · Human Serome Atlas

MTIF2

Translation initiation factor IF-2, mitochondrial

Also known as: IF-2mt, IF2M_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P46199
Gene: MTIF2
Ensembl: ENSG00000085760
Chromosome: 2
Canonical length: 727 aa
Protein class: Predicted intracellular proteins
Subcellular location: Mitochondria

OverviewNCBI Gene

During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. [provided by RefSeq, Mar 2016]

Canonical amino-acid sequenceUniProt

727 residues, UniProt reviewed canonical sequence.

>P46199|MTIF2
     1  MNQKLLKLEN LLRFHTIYRQ LHSLCQRRAL RQWRHGFSSA YPVWTAQLCA WPWPTDVLTG
    61  AALSQYRLLV TKKEEGPWKS QLSSTKSKKV VEVWIGMTIE ELARAMEKNT DYVYEALLNT
   121  DIDIDSLEAD SHLDEVWIKE VITKAGMKLK WSKLKQDKVR KNKDAVRRPQ ADPALLTPRS
   181  PVVTIMGHVD HGKTTLLDKF RKTQVAAVET GGITQHIGAF LVSLPSGEKI TFLDTPGHAA
   241  FSAMRARGAQ VTDIVVLVVA ADDGVMKQTV ESIQHAKDAQ VPIILAVNKC DKAEADPEKV
   301  KKELLAYDVV CEDYGGDVQA VPVSALTGDN LMALAEATVA LAEMLELKAD PNGPVEGTVI
   361  ESFTDKGRGL VTTAIIQRGT LRKGSVLVAG KCWAKVRLMF DENGKTIDEA YPSMPVGITG
   421  WRDLPSAGEE ILEVESEPRA REVVDWRKYE QEQEKGQEDL KIIEEKRKEH KEAHQKAREK
   481  YGHLLWKKRS ILRFLERKEQ IPLKPKEKRE RDSNVLSVII KGDVDGSVEA ILNIIDTYDA
   541  SHECELELVH FGVGDVSAND VNLAETFDGV IYGFNVNAGN VIQQSAAKKG VKIKLHKIIY
   601  RLVEDLQEEL SSRLPCAVEE HPVGEASILA TFSVTEGKKK VPVAGCRVQK GQLEKQKKFK
   661  LTRNGHVIWK GSLTSLKHHK DDISIVKTGM DCGLSLDEDN MEFQVGDRIV CYEEKQIQAK
   721  TSWDPGF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MTIF2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.33
Highest tissue expression: 46 nTPM

Expression across tissuesHPA

Tissue

tongue: 46 nTPM
skeletal muscle: 41 nTPM
liver: 31 nTPM
heart muscle: 30 nTPM
rectum: 30 nTPM
colon: 26 nTPM

Single-cell type

thymic myoid cells: 56 nCPM
erythrocyte progenitors: 55 nCPM
esophageal basal cells: 55 nCPM
megakaryocyte-erythroid progenitors: 50 nCPM
esophageal suprabasal cells: 50 nCPM
myonuclei: 50 nCPM

Immune cell

NK-cell: 17 nTPM
myeloid DC: 14 nTPM
intermediate monocyte: 13 nTPM
T-reg: 12 nTPM
MAIT T-cell: 12 nTPM
naive CD8 T-cell: 12 nTPM

Brain region

choroid plexus: 29 nTPM
white matter: 26 nTPM
hypothalamus: 25 nTPM
thalamus: 25 nTPM
cerebellum: 24 nTPM
medulla oblongata: 24 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MTIF2.

Disease | GeneticClinVar

2 pathogenic / likely-pathogenic of 129 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Inborn genetic diseases

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.84
gnomAD pLI: 0
gnomAD missense Z: -0.3
DepMap mean gene effect: -0.35
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Translational (tr)-type GTP-binding domain
Small GTP-binding domain
Translation protein, beta-barrel domain superfamily
Translation initiation factor IF- 2
Translation initiation factor IF- 2, domain 3
P-loop containing nucleoside triphosphate hydrolase
Translation initiation factor IF-2, domain 3 superfamily
Elongation factor G-like, domain II
Elongation factor Tu GTP binding domain
Translation-initiation factor 2
Elongation factor G domain 2
Translation initiation factor IF-2, bacterial-like
Translation initiation factor IF-2, domain II

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MTIF2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MTIF2 as an antibody target. Whether an autoantibody or antibody against MTIF2 could matter depends on whether native MTIF2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MTIF2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MTIF2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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