AK4

Protein identityUniProt · HPA

UniProt accession

P27144

Gene

AK4

Ensembl

ENSG00000162433

Chromosome

1

Canonical length

223 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Mitochondria

OverviewNCBI Gene

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

223 residues, UniProt reviewed canonical sequence.

>P27144|AK4
     1  MASKLLRAVI LGPPGSGKGT VCQRIAQNFG LQHLSSGHFL RENIKASTEV GEMAKQYIEK
    61  SLLVPDHVIT RLMMSELENR RGQHWLLDGF PRTLGQAEAL DKICEVDLVI SLNIPFETLK
   121  DRLSRRWIHP PSGRVYNLDF NPPHVHGIDD VTGEPLVQQE DDKPEAVAAR LRQYKDVAKP
   181  VIELYKSRGV LHQFSGTETN KIWPYVYTLF SNKITPIQSK EAY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against AK4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.27

Highest tissue expression

169 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.79

gnomAD pLI

0.13

gnomAD missense Z

1.23

DepMap mean gene effect

-0.29

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• ADP biosynthetic process
• AMP metabolic process
• ATP metabolic process
• cellular response to hypoxia
• GTP metabolic process
• nucleobase-containing small molecule interconversion
• nucleoside triphosphate biosynthetic process
• regulation of oxidative phosphorylation
• ribonucleoside diphosphate biosynthetic process

Molecular functions

• AMP kinase activity
• ATP binding
• CMP kinase activity
• dAMP kinase activity
• dCMP kinase activity
• GTP binding
• nucleoside diphosphate kinase activity
• nucleoside monophosphate kinase activity
• nucleoside triphosphate adenylate kinase activity

Cellular components

• cytoplasm
• mitochondrial matrix
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Adenylate kinase/UMP-CMP kinase
• Adenylate kinase subfamily
• Adenylate kinase, active site lid domain
• P-loop containing nucleoside triphosphate hydrolase
• Adenylate kinase 3/4, mitochondrial
• Adenylate kinase, conserved site
• Adenylate kinase, active site lid domain superfamily
• Adenylate kinase
• Adenylate kinase, active site lid
• Adenylate kinase 4, mitochondrial

KeywordsUniProt

• Acetylation
• ATP-binding
• GTP-binding
• Kinase
• Mitochondrion
• Nucleotide-binding
• Transferase

InteractionsUniProt · HPA

Protein binding partners of AK4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• SLC25A5
• MTG2
• MTIF2