Seroatlas · Human Serome Atlas

MRPS25

Small ribosomal subunit protein mS25

Also known as: DKFZp313H0817, FLJ00023, MRP-S25, RPMS25, RT25_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P82663
Gene
MRPS25
Ensembl
ENSG00000131368
Chromosome
3
Canonical length
173 aa
Protein class
Disease related genes, Human disease related genes, Predicted intracellular proteins, Ribosomal proteins
Subcellular location
Mitochondria

OverviewNCBI Gene

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Canonical amino-acid sequenceUniProt

173 residues, UniProt reviewed canonical sequence.

>P82663|MRPS25
     1  MPMKGRFPIR RTLQYLSQGN VVFKDSVKVM TVNYNTHGEL GEGARKFVFF NIPQIQYKNP
    61  WVQIMMFKNM TPSPFLRFYL DSGEQVLVDV ETKSNKEIME HIRKILGKNE ETLREEEEEK
   121  KQLSHPANFG PRKYCLRECI CEVEGQVPCP SLVPLPKEMR GKYKAALKAD AQD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MRPS25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
64 nTPM

Expression across tissuesHPA

Tissue

  • heart muscle: 64 nTPM
  • skeletal muscle: 52 nTPM
  • liver: 44 nTPM
  • thyroid gland: 42 nTPM
  • testis: 34 nTPM
  • cerebellum: 34 nTPM

Single-cell type

  • epicardial cells: 216 nCPM
  • cardiomyocytes: 142 nCPM
  • late spermatids: 141 nCPM
  • choroid plexus epithelial cells: 118 nCPM
  • myonuclei: 102 nCPM
  • early spermatids: 87 nCPM

Immune cell

  • memory B-cell: 65 nTPM
  • naive B-cell: 51 nTPM
  • non-classical monocyte: 47 nTPM
  • intermediate monocyte: 42 nTPM
  • classical monocyte: 37 nTPM
  • naive CD4 T-cell: 35 nTPM

Brain region

  • pons: 30 nTPM
  • choroid plexus: 23 nTPM
  • white matter: 23 nTPM
  • cerebral cortex: 21 nTPM
  • basal ganglia: 20 nTPM
  • thalamus: 20 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MRPS25.

Disease | AllUniProt

Conditions MRPS25 is implicated in, by any mechanism.

  • Combined oxidative phosphorylation deficiency 50 (COXPD50) MIM:619025

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 40 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Combined oxidative phosphorylation deficiency 50

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.38
gnomAD pLI
0
gnomAD missense Z
0.81
DepMap mean gene effect
-0.44
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MRPS25 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MRPS25 as an antibody target. Whether an autoantibody or antibody against MRPS25 could matter depends on whether native MRPS25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MRPS25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MRPS25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MRPS25. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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