Seroatlas · Human Serome Atlas

MRPS35

Small ribosomal subunit protein mS35

Also known as: MDS023, MRPS28, RT35_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P82673
Gene
MRPS35
Ensembl
ENSG00000061794
Chromosome
12
Canonical length
323 aa
Protein class
Predicted intracellular proteins, Ribosomal proteins
Subcellular location
Mitochondria,Cytosol,Connecting piece

OverviewNCBI Gene

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q. [provided by RefSeq, Jul 2010]

Canonical amino-acid sequenceUniProt

323 residues, UniProt reviewed canonical sequence.

>P82673|MRPS35
     1  MAAAALPAWL SLQSRARTLR AFSTAVYSAT PVPTPSLPER TPGNERPPRR KALPPRTEKM
    61  AVDQDWPSVY PVAAPFKPSA VPLPVRMGYP VKKGVPMAKE GNLELLKIPN FLHLTPVAIK
   121  KHCEALKDFC TEWPAALDSD EKCEKHFPIE IDSTDYVSSG PSVRNPRARV VVLRVKLSSL
   181  NLDDHAKKKL IKLVGERYCK TTDVLTIKTD RCPLRRQNYD YAVYLLTVLY HESWNTEEWE
   241  KSKTEADMEE YIWENSSSER NILETLLQMK AAEKNMEINK EELLGTKEIE EYKKSVVSLK
   301  NEEENENSIS QYKESVKRLL NVT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MRPS35 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.45
Highest tissue expression
78 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 78 nTPM
  • kidney: 73 nTPM
  • liver: 73 nTPM
  • tongue: 68 nTPM
  • heart muscle: 58 nTPM
  • rectum: 57 nTPM

Single-cell type

  • parietal cells: 175 nCPM
  • migrating cytotrophoblasts: 160 nCPM
  • esophageal basal cells: 152 nCPM
  • esophageal suprabasal cells: 148 nCPM
  • gastric progenitor cells: 145 nCPM
  • extravillous trophoblasts: 141 nCPM

Immune cell

  • non-classical monocyte: 47 nTPM
  • intermediate monocyte: 38 nTPM
  • myeloid DC: 31 nTPM
  • T-reg: 27 nTPM
  • NK-cell: 26 nTPM
  • MAIT T-cell: 24 nTPM

Brain region

  • choroid plexus: 28 nTPM
  • white matter: 26 nTPM
  • hypothalamus: 26 nTPM
  • spinal cord: 25 nTPM
  • cerebellum: 25 nTPM
  • pons: 24 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.25
gnomAD pLI
0
gnomAD missense Z
-0.3
DepMap mean gene effect
-0.48
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Small ribosomal subunit protein mS35, mitochondrial, conserved domain
  • Small ribosomal subunit protein mS35, mitochondrial
  • Mitochondrial ribosomal subunit protein

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MRPS35 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MRPS35 as an antibody target. Whether an autoantibody or antibody against MRPS35 could matter depends on whether native MRPS35 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MRPS35 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MRPS35 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MRPS35. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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