MRPS12

Protein identityUniProt · HPA

UniProt accession

O15235

Gene

MRPS12

Ensembl

ENSG00000128626

Chromosome

19

Canonical length

138 aa

Protein class

Predicted intracellular proteins, Ribosomal proteins

OverviewNCBI Gene

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

138 residues, UniProt reviewed canonical sequence.

>O15235|MRPS12
     1  MSWSGLLHGL NTSLTCGPAL VPRLWATCSM ATLNQMHRLG PPKRPPRKLG PTEGRPQLKG
    61  VVLCTFTRKP KKPNSANRKC CRVRLSTGRE AVCFIPGEGH TLQEHQIVLV EGGRTQDLPG
   121  VKLTVVRGKY DCGHVQKK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MRPS12 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.47

Highest tissue expression

73 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MRPS12.

Disease | ImmuneIEDB

Conditions an epitope on MRPS12 was assayed in.

• colonic benign neoplasm T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.89

gnomAD pLI

0

gnomAD missense Z

0.42

DepMap mean gene effect

-0.55

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• mitochondrial translation
• translation

Molecular functions

• RNA binding
• structural constituent of ribosome

Cellular components

• mitochondrial inner membrane
• mitochondrial ribosome
• mitochondrial small ribosomal subunit
• mitochondrion
• ribosome

Protein domainsUniProt · Pfam · InterPro

• Small ribosomal subunit protein uS12
• Nucleic acid-binding, OB-fold
• Ribosomal protein S12/S23
• Ribosomal protein uS12, bacteria

KeywordsUniProt

• Mitochondrion
• Ribonucleoprotein
• Ribosomal protein
• Transit peptide

InteractionsUniProt · HPA

Protein binding partners of MRPS12 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MRPS17
• MRPS25
• MRPS26
• MRPS34
• SPINK7