Seroatlas · Human Serome Atlas

GNAI3

Guanine nucleotide-binding protein G(i) subunit alpha-3

Also known as: 87U6, GNAI3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P08754
Gene
GNAI3
Ensembl
ENSG00000065135
Chromosome
1
Canonical length
354 aa
Protein class
Disease related genes, Human disease related genes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nucleoli,Golgi apparatus,Centriolar satellite,Centrosome,Basal body,Cytosol,Principal piece

OverviewNCBI Gene

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012]

Canonical amino-acid sequenceUniProt

354 residues, UniProt reviewed canonical sequence.

>P08754|GNAI3
     1  MGCTLSAEDK AAVERSKMID RNLREDGEKA AKEVKLLLLG AGESGKSTIV KQMKIIHEDG
    61  YSEDECKQYK VVVYSNTIQS IIAIIRAMGR LKIDFGEAAR ADDARQLFVL AGSAEEGVMT
   121  PELAGVIKRL WRDGGVQACF SRSREYQLND SASYYLNDLD RISQSNYIPT QQDVLRTRVK
   181  TTGIVETHFT FKDLYFKMFD VGGQRSERKK WIHCFEGVTA IIFCVALSDY DLVLAEDEEM
   241  NRMHESMKLF DSICNNKWFT ETSIILFLNK KDLFEEKIKR SPLTICYPEY TGSNTYEEAA
   301  AYIQCQFEDL NRRKDTKEIY THFTCATDTK NVQFVFDAVT DVIIKNNLKE CGLY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GNAI3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.26
Highest tissue expression
26 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 26 nTPM
  • esophagus: 21 nTPM
  • skin: 17 nTPM
  • retina: 17 nTPM
  • tonsil: 15 nTPM
  • urinary bladder: 15 nTPM

Single-cell type

  • neutrophils: 1,086 nCPM
  • esophageal apical cells: 760 nCPM
  • neutrophil progenitors: 342 nCPM
  • esophageal suprabasal cells: 335 nCPM
  • monocytes: 253 nCPM
  • suprabasal keratinocytes: 241 nCPM

Immune cell

  • eosinophil: 58 nTPM
  • neutrophil: 54 nTPM
  • non-classical monocyte: 45 nTPM
  • intermediate monocyte: 44 nTPM
  • total PBMC: 36 nTPM
  • basophil: 33 nTPM

Brain region

  • choroid plexus: 15 nTPM
  • cerebellum: 12 nTPM
  • white matter: 12 nTPM
  • medulla oblongata: 11 nTPM
  • hypothalamus: 10 nTPM
  • thalamus: 9.9 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about GNAI3.

Disease | AllUniProt

Conditions GNAI3 is implicated in, by any mechanism.

Disease | GeneticClinVar

8 pathogenic / likely-pathogenic of 104 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.61
gnomAD pLI
0.04
gnomAD missense Z
1.79
DepMap mean gene effect
-0.03
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of GNAI3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads GNAI3 as an antibody target. Whether an autoantibody or antibody against GNAI3 could matter depends on whether native GNAI3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

GNAI3 is annotated at the cell surface, where native GNAI3 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label GNAI3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/GNAI3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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