Seroatlas · Human Serome Atlas

EIF5

Eukaryotic translation initiation factor 5

Also known as: IF5_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P55010
Gene
EIF5
Ensembl
ENSG00000100664
Chromosome
14
Canonical length
431 aa
Protein class
Predicted intracellular proteins
Subcellular location
Cytosol

OverviewNCBI Gene

Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010]

Canonical amino-acid sequenceUniProt

431 residues, UniProt reviewed canonical sequence.

>P55010|EIF5
     1  MSVNVNRSVS DQFYRYKMPR LIAKVEGKGN GIKTVIVNMV DVAKALNRPP TYPTKYFGCE
    61  LGAQTQFDVK NDRYIVNGSH EANKLQDMLD GFIKKFVLCP ECENPETDLH VNPKKQTIGN
   121  SCKACGYRGM LDTHHKLCTF ILKNPPENSD SGTGKKEKEK KNRKGKDKEN GSVSSSETPP
   181  PPPPPNEINP PPHTMEEEED DDWGEDTTEE AQRRRMDEIS DHAKVLTLSD DLERTIEERV
   241  NILFDFVKKK KEEGVIDSSD KEIVAEAERL DVKAMGPLVL TEVLFNEKIR EQIKKYRRHF
   301  LRFCHNNKKA QRYLLHGLEC VVAMHQAQLI SKIPHILKEM YDADLLEEEV IISWSEKASK
   361  KYVSKELAKE IRVKAEPFIK WLKEAEEESS GGEEEDEDEN IEVVYSKAAS VPKVETVKSD
   421  NKDDDIDIDA I

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EIF5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.39
Highest tissue expression
184 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 184 nTPM
  • skeletal muscle: 153 nTPM
  • liver: 141 nTPM
  • tongue: 135 nTPM
  • testis: 89 nTPM
  • thyroid gland: 88 nTPM

Single-cell type

  • late spermatids: 1,163 nCPM
  • esophageal apical cells: 835 nCPM
  • epididymal basal cells: 649 nCPM
  • syncytiotrophoblasts: 634 nCPM
  • late primary spermatocytes: 565 nCPM
  • esophageal suprabasal cells: 486 nCPM

Immune cell

  • total PBMC: 396 nTPM
  • basophil: 351 nTPM
  • memory B-cell: 328 nTPM
  • naive B-cell: 328 nTPM
  • plasmacytoid DC: 324 nTPM
  • eosinophil: 320 nTPM

Brain region

  • white matter: 126 nTPM
  • spinal cord: 114 nTPM
  • hypothalamus: 106 nTPM
  • midbrain: 104 nTPM
  • pons: 103 nTPM
  • cerebellum: 100 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.28
gnomAD pLI
0.99
gnomAD missense Z
1.56
DepMap mean gene effect
-1.4
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EIF5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EIF5 as an antibody target. Whether an autoantibody or antibody against EIF5 could matter depends on whether native EIF5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EIF5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EIF5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EIF5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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