Seroatlas · Human Serome Atlas

EHD3

EH domain-containing protein 3

Also known as: EHD3_HUMAN, PAST3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9NZN3
Gene
EHD3
Ensembl
ENSG00000013016
Chromosome
2
Canonical length
535 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Plasma membrane,Primary cilium,Primary cilium transition zone
Quaternary structure
Homooligomer

OverviewNCBI Gene

Predicted to enable nucleic acid binding activity. Involved in several processes, including cytosolic transport; endocytic recycling; and protein homooligomerization. Acts upstream of or within protein localization to plasma membrane and regulation of cardiac muscle cell membrane potential. Located in ciliary pocket membrane and recycling endosome membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

535 residues, UniProt reviewed canonical sequence.

>Q9NZN3|EHD3
     1  MFSWLGTDDR RRKDPEVFQT VSEGLKKLYK SKLLPLEEHY RFHEFHSPAL EDADFDNKPM
    61  VLLVGQYSTG KTTFIRYLLE QDFPGMRIGP EPTTDSFIAV MQGDMEGIIP GNALVVDPKK
   121  PFRKLNAFGN AFLNRFVCAQ LPNPVLESIS VIDTPGILSG EKQRISRGYD FAAVLEWFAE
   181  RVDRIILLFD AHKLDISDEF SEVIKALKNH EDKMRVVLNK ADQIETQQLM RVYGALMWSL
   241  GKIVNTPEVI RVYIGSFWSH PLLIPDNRKL FEAEEQDLFR DIQSLPRNAA LRKLNDLIKR
   301  ARLAKVHAYI ISSLKKEMPS VFGKDNKKKE LVNNLAEIYG RIEREHQISP GDFPNLKRMQ
   361  DQLQAQDFSK FQPLKSKLLE VVDDMLAHDI AQLMVLVRQE ESQRPIQMVK GGAFEGTLHG
   421  PFGHGYGEGA GEGIDDAEWV VARDKPMYDE IFYTLSPVDG KITGANAKKE MVRSKLPNSV
   481  LGKIWKLADI DKDGMLDDDE FALANHLIKV KLEGHELPNE LPAHLLPPSK RKVAE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EHD3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.27
Highest tissue expression
41 nTPM

Expression across tissuesHPA

Tissue

  • cerebral cortex: 41 nTPM
  • esophagus: 41 nTPM
  • hippocampal formation: 28 nTPM
  • amygdala: 28 nTPM
  • blood vessel: 26 nTPM
  • cerebellum: 21 nTPM

Single-cell type

  • esophageal apical cells: 332 nCPM
  • platelets: 273 nCPM
  • megakaryocytes: 105 nCPM
  • syncytiotrophoblasts: 88 nCPM
  • ocular epithelial cells: 68 nCPM
  • oligodendrocyte progenitor cells: 59 nCPM

Immune cell

  • memory B-cell: 21 nTPM
  • total PBMC: 10 nTPM
  • naive B-cell: 5.6 nTPM
  • non-classical monocyte: 2.8 nTPM
  • gdT-cell: 2.1 nTPM
  • memory CD8 T-cell: 1.4 nTPM

Brain region

  • cerebral cortex: 99 nTPM
  • thalamus: 85 nTPM
  • hippocampal formation: 79 nTPM
  • white matter: 72 nTPM
  • basal ganglia: 67 nTPM
  • hypothalamus: 59 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.6
gnomAD pLI
0.04
gnomAD missense Z
1.82
DepMap mean gene effect
0.02
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EHD3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EHD3 as an antibody target. Whether an autoantibody or antibody against EHD3 could matter depends on whether native EHD3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EHD3 is annotated at the cell surface, where native EHD3 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label EHD3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EHD3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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