Seroatlas · Human Serome Atlas

DHX8

ATP-dependent RNA helicase DHX8

Also known as: DDX8, Dhr2, DHX8_HUMAN, HRH1, PRP22, PRPF22

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q14562
Gene: DHX8
Ensembl: ENSG00000067596
Chromosome: 17
Canonical length: 1220 aa
Protein class: Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear bodies

OverviewNCBI Gene

This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Canonical amino-acid sequenceUniProt

1220 residues, UniProt reviewed canonical sequence.

>Q14562|DHX8
     1  MAVAVAMAGA LIGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA EFVISLAEKN
    61  TTFDTFKASL VKNGAEFTDS LISNLLRLIQ TMRPPAKPST SKDPVVKPKT EKEKLKELFP
   121  VLCQPDNPSV RTMLDEDDVK VAVDVLKELE ALMPSAAGQE KQRDAEHRDR TKKKKRSRSR
   181  DRNRDRDRDR ERNRDRDHKR RHRSRSRSRS RTRERNKVKS RYRSRSRSQS PPKDRKDRDK
   241  YGERNLDRWR DKHVDRPPPE EPTIGDIYNG KVTSIMQFGC FVQLEGLRKR WEGLVHISEL
   301  RREGRVANVA DVVSKGQRVK VKVLSFTGTK TSLSMKDVDQ ETGEDLNPNR RRNLVGETNE
   361  ETSMRNPDRP THLSLVSAPE VEDDSLERKR LTRISDPEKW EIKQMIAANV LSKEEFPDFD
   421  EETGILPKVD DEEDEDLEIE LVEEEPPFLR GHTKQSMDMS PIKIVKNPDG SLSQAAMMQS
   481  ALAKERRELK QAQREAEMDS IPMGLNKHWV DPLPDAEGRQ IAANMRGIGM MPNDIPEWKK
   541  HAFGGNKASY GKKTQMSILE QRESLPIYKL KEQLVQAVHD NQILIVIGET GSGKTTQITQ
   601  YLAEAGYTSR GKIGCTQPRR VAAMSVAKRV SEEFGCCLGQ EVGYTIRFED CTSPETVIKY
   661  MTDGMLLREC LIDPDLTQYA IIMLDEAHER TIHTDVLFGL LKKTVQKRQD MKLIVTSATL
   721  DAVKFSQYFY EAPIFTIPGR TYPVEILYTK EPETDYLDAS LITVMQIHLT EPPGDILVFL
   781  TGQEEIDTAC EILYERMKSL GPDVPELIIL PVYSALPSEM QTRIFDPAPP GSRKVVIATN
   841  IAETSLTIDG IYYVVDPGFV KQKVYNSKTG IDQLVVTPIS QAQAKQRAGR AGRTGPGKCY
   901  RLYTERAYRD EMLTTNVPEI QRTNLASTVL SLKAMGINDL LSFDFMDAPP METLITAMEQ
   961  LYTLGALDDE GLLTRLGRRM AEFPLEPMLC KMLIMSVHLG CSEEMLTIVS MLSVQNVFYR
  1021  PKDKQALADQ KKAKFHQTEG DHLTLLAVYN SWKNNKFSNP WCYENFIQAR SLRRAQDIRK
  1081  QMLGIMDRHK LDVVSCGKST VRVQKAICSG FFRNAAKKDP QEGYRTLIDQ QVVYIHPSSA
  1141  LFNRQPEWVV YHELVLTTKE YMREVTTIDP RWLVEFAPAF FKVSDPTKLS KQKKQQRLEP
  1201  LYNRYEEPNA WRISRAFRRR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DHX8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.34
Highest tissue expression: 51 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 51 nTPM
thymus: 32 nTPM
skin: 31 nTPM
tonsil: 27 nTPM
esophagus: 26 nTPM
parathyroid gland: 25 nTPM

Single-cell type

neutrophils: 101 nCPM
neutrophil progenitors: 62 nCPM
neuroendocrine cells: 59 nCPM
retinal bipolar cells: 55 nCPM
rod photoreceptor cells: 55 nCPM
cone photoreceptor cells: 54 nCPM

Immune cell

basophil: 30 nTPM
non-classical monocyte: 26 nTPM
intermediate monocyte: 24 nTPM
neutrophil: 22 nTPM
eosinophil: 20 nTPM
classical monocyte: 17 nTPM

Brain region

thalamus: 22 nTPM
cerebral cortex: 22 nTPM
pons: 21 nTPM
amygdala: 21 nTPM
basal ganglia: 21 nTPM
medulla oblongata: 21 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.5
gnomAD pLI: 0
gnomAD missense Z: 5.03
DepMap mean gene effect: -1.54
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DHX8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DHX8 as an antibody target. Whether an autoantibody or antibody against DHX8 could matter depends on whether native DHX8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DHX8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DHX8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...