Seroatlas · Human Serome Atlas

ACTR3

Actin-related protein 3

Also known as: ARP3, ARP3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P61158
Gene: ACTR3
Ensembl: ENSG00000115091
Chromosome: 2
Canonical length: 418 aa
Protein class: Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]

Canonical amino-acid sequenceUniProt

418 residues, UniProt reviewed canonical sequence.

>P61158|ACTR3
     1  MAGRLPACVV DCGTGYTKLG YAGNTEPQFI IPSCIAIKES AKVGDQAQRR VMKGVDDLDF
    61  FIGDEAIEKP TYATKWPIRH GIVEDWDLME RFMEQVIFKY LRAEPEDHYF LLTEPPLNTP
   121  ENREYTAEIM FESFNVPGLY IAVQAVLALA ASWTSRQVGE RTLTGTVIDS GDGVTHVIPV
   181  AEGYVIGSCI KHIPIAGRDI TYFIQQLLRD REVGIPPEQS LETAKAVKER YSYVCPDLVK
   241  EFNKYDTDGS KWIKQYTGIN AISKKEFSID VGYERFLGPE IFFHPEFANP DFTQPISEVV
   301  DEVIQNCPID VRRPLYKNIV LSGGSTMFRD FGRRLQRDLK RTVDARLKLS EELSGGRLKP
   361  KPIDVQVITH HMQRYAVWFG GSMLASTPEF YQVCHTKKDY EEIGPSICRH NPVFGVMS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ACTR3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.25
Highest tissue expression: 310 nTPM

Expression across tissuesHPA

Tissue

tonsil: 310 nTPM
lymph node: 272 nTPM
appendix: 217 nTPM
bone marrow: 205 nTPM
thymus: 173 nTPM
esophagus: 157 nTPM

Single-cell type

neutrophils: 2,891 nCPM
neutrophil progenitors: 1,359 nCPM
megakaryocytes: 1,193 nCPM
esophageal apical cells: 1,048 nCPM
monocytes: 575 nCPM
esophageal suprabasal cells: 513 nCPM

Immune cell

total PBMC: 1,267 nTPM
eosinophil: 1,052 nTPM
non-classical monocyte: 894 nTPM
neutrophil: 782 nTPM
basophil: 756 nTPM
intermediate monocyte: 730 nTPM

Brain region

choroid plexus: 131 nTPM
medulla oblongata: 121 nTPM
hippocampal formation: 120 nTPM
white matter: 115 nTPM
hypothalamus: 109 nTPM
thalamus: 106 nTPM

ReferencesPubMed · IEDB

Publications for ACTR3 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

1 publication

Plasma IgG autoantibody against actin-related protein 3 in liver fluke Opisthorchis viverrini infection.
2015 · Parasite Immunol · RCR 0.3 · 8 citations

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.12
gnomAD pLI: 1
gnomAD missense Z: 3.61
DepMap mean gene effect: -0.56
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ACTR3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ACTR3 as an antibody target. Whether an autoantibody or antibody against ACTR3 could matter depends on whether native ACTR3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ACTR3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ACTR3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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