YTHDC2

Protein identityUniProt · HPA

UniProt accession

Q9H6S0

Gene

YTHDC2

Ensembl

ENSG00000047188

Chromosome

5

Canonical length

1430 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nuclear bodies,Cytoplasmic bodies

OverviewNCBI Gene

This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line. [provided by RefSeq, Sep 2016]

Canonical amino-acid sequenceUniProt

1430 residues, UniProt reviewed canonical sequence.

>Q9H6S0|YTHDC2
     1  MSRPSSVSPR QPAPGGGGGG GPSPCGPGGG GRAKGLKDIR IDEEVKIAVN IALERFRYGD
    61  QREMEFPSSL TSTERAFIHR LSQSLGLVSK SKGKGANRYL TVKKKDGSET AHAMMTCNLT
   121  HNTKHAVRSL IQRFPVTNKE RTELLPKTER GNVFAVEAEN REMSKTSGRL NNGIPQIPVK
   181  RGESEFDSFR QSLPVFEKQE EIVKIIKENK VVLIVGETGS GKTTQIPQFL LDDCFKNGIP
   241  CRIFCTQPRR LAAIAVAERV AAERRERIGQ TIGYQIRLES RVSPKTLLTF CTNGVLLRTL
   301  MAGDSTLSTV THVIVDEVHE RDRFSDFLLT KLRDLLQKHP TLKLILSSAA LDVNLFIRYF
   361  GSCPVIYIQG RPFEVKEMFL EDILRTTGYT NKEMLKYKKE KQQEEKQQTT LTEWYSAQEN
   421  SFKPESQRQR TVLNVTDEYD LLDDGGDAVF SQLTEKDVNC LEPWLIKEMD ACLSDIWLHK
   481  DIDAFAQVFH LILTENVSVD YRHSETSATA LMVAAGRGFA SQVEQLISMG ANVHSKASNG
   541  WMALDWAKHF GQTEIVDLLE SYSATLEFGN LDESSLVQTN GSDLSAEDRE LLKAYHHSFD
   601  DEKVDLDLIM HLLYNICHSC DAGAVLIFLP GYDEIVGLRD RILFDDKRFA DSTHRYQVFM
   661  LHSNMQTSDQ KKVLKNPPAG VRKIILSTNI AETSITVNDV VFVIDSGKVK EKSFDALNFV
   721  TMLKMVWISK ASAIQRKGRA GRCRPGICFR LFSRLRFQNM LEFQTPELLR MPLQELCLHT
   781  KLLAPVNCPI ADFLMKAPEP PPALIVRNAV QMLKTIDAMD TWEDLTELGY HLADLPVEPH
   841  LGKMVLCAVV LKCLDPILTI ACTLAYRDPF VLPTQASQKR AAMLCRKRFT AGAFSDHMAL
   901  LRAFQAWQKA RSDGWERAFC EKNFLSQATM EIIIGMRTQL LGQLRASGFV RARGGGDIRD
   961  VNTNSENWAV VKAALVAGMY PNLVHVDREN LVLTGPKEKK VRFHPASVLS QPQYKKIPPA
  1021  NGQAAAIKAL PTDWLIYDEM TRAHRIANIR CCSAVTPVTI LVFCGPARLA SNALQEPSSF
  1081  RVDGIPNDSS DSEMEDKTTA NLAALKLDEW LHFTLEPEAA SLLLQLRQKW HSLFLRRMRA
  1141  PSKPWSQVDE ATIRAIIAVL STEEQSAGLQ QPSGIGQRPR PMSSEELPLA SSWRSNNSRK
  1201  SSADTEFSDE CTTAERVLMK SPSPALHPPQ KYKDRGILHP KRGTEDRSDQ SSLKSTDSSS
  1261  YPSPCASPSP PSSGKGSKSP SPRPNMPVRY FIMKSSNLRN LEISQQKGIW STTPSNERKL
  1321  NRAFWESSIV YLVFSVQGSG HFQGFSRMSS EIGREKSQDW GSAGLGGVFK VEWIRKESLP
  1381  FQFAHHLLNP WNDNKKVQIS RDGQELEPLV GEQLLQLWER LPLGEKNTTD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against YTHDC2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.31

Highest tissue expression

17 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.13

gnomAD pLI

1

gnomAD missense Z

0.33

DepMap mean gene effect

0

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• germline cell cycle switching, mitotic to meiotic cell cycle
• host-mediated activation of viral genome replication
• meiotic cell cycle
• oocyte development
• response to interleukin-1
• response to tumor necrosis factor
• spermatid development

Molecular functions

• 3'-5' RNA helicase activity
• ATP binding
• ATP hydrolysis activity
• ATP-dependent activity, acting on RNA
• helicase activity
• N6-methyladenosine-containing RNA reader activity
• RNA binding
• RNA polymerase binding

Cellular components

• endoplasmic reticulum
• nucleoplasm
• perinuclear region of cytoplasm
• ribonucleoprotein granule

Protein domainsUniProt · Pfam · InterPro

• R3H domain
• Helicase, C-terminal domain-like
• Ankyrin repeat
• YTH domain
• Helicase-associated domain
• DEAD/DEAH-box helicase domain
• DEAD-box helicase, OB fold
• Helicase superfamily 1/2, ATP-binding domain
• P-loop containing nucleoside triphosphate hydrolase
• Ankyrin repeat-containing domain superfamily
• R3H domain superfamily
• Helicase associated domain (HA2), winged-helix domain
• RNA helicase, C-terminal domain
• DEAD/DEAH box helicase
• Helicase conserved C-terminal domain
• R3H domain
• YT521-B-like domain
• Helicase associated domain (HA2), winged-helix
• Oligonucleotide/oligosaccharide-binding (OB)-fold
• Helicase associated domain (HA2), ratchet-like
• C-terminal domain in RNA helicases
• DExH-box ATP-dependent RNA helicase, R3H domain

KeywordsUniProt

• ANK repeat
• ATP-binding
• Cytoplasm
• Differentiation
• Helicase
• Hydrolase
• Meiosis
• Nucleotide-binding
• Oogenesis
• Phosphoprotein
• Repeat
• RNA-binding
• Spermatogenesis

InteractionsUniProt · HPA

Protein binding partners of YTHDC2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MEIOC
• RBM46
• ZCCHC4
• XRN1
• IRF4
• RPS16