Seroatlas · Human Serome Atlas

ZCCHC4

rRNA N(6)-adenosine-methyltransferase ZCCHC4

Also known as: FLJ23024, HSPC052, ZCHC4_HUMAN, ZGRF4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9H5U6
Gene
ZCCHC4
Ensembl
ENSG00000168228
Chromosome
4
Canonical length
513 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nuclear speckles,Golgi apparatus

OverviewNCBI Gene

Enables S-adenosyl-L-methionine binding activity; rRNA (adenine-N6-)-methyltransferase activity; and zinc ion binding activity. Involved in positive regulation of translation and rRNA methylation. Located in cytoplasm and nucleolus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

513 residues, UniProt reviewed canonical sequence.

>Q9H5U6|ZCCHC4
     1  MAASRNGFEA VEAEGSAGCR GSSGMEVVLP LDPAVPAPLC PHGPTLLFVK VTQGKEETRR
    61  FYACSACRDR KDCNFFQWED EKLSGARLAA REAHNRRCQP PLSRTQCVER YLKFIELPLT
   121  QRKFCQTCQQ LLLPDDWGQH SEHQVLGNVS ITQLRRPSQL LYPLENKKTN AQYLFADRSC
   181  QFLVDLLSAL GFRRVLCVGT PRLHELIKLT ASGDKKSNIK SLLLDIDFRY SQFYMEDSFC
   241  HYNMFNHHFF DGKTALEVCR AFLQEDKGEG IIMVTDPPFG GLVEPLAITF KKLIAMWKEG
   301  QSQDDSHKEL PIFWIFPYFF ESRICQFFPS FQMLDYQVDY DNHALYKHGK TGRKQSPVRI
   361  FTNIPPNKII LPTEEGYRFC SPCQRYVSLE NQHCELCNSC TSKDGRKWNH CFLCKKCVKP
   421  SWIHCSICNH CAVPDHSCEG PKHGCFICGE LDHKRSTCPN IATSKRANKA VRKQKQRKSN
   481  KMKMETTKGQ SMNHTSATRR KKRRERAHQY LGS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZCCHC4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.33
Highest tissue expression
6.7 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 6.7 nTPM
  • parathyroid gland: 5.6 nTPM
  • tongue: 5.2 nTPM
  • ovary: 5.1 nTPM
  • breast: 4.7 nTPM
  • endometrium: 4.7 nTPM

Single-cell type

  • myonuclei: 45 nCPM
  • retinal horizontal cells: 30 nCPM
  • sertoli cells: 28 nCPM
  • choroid plexus epithelial cells: 27 nCPM
  • cardiomyocytes: 25 nCPM
  • basal prostatic cells: 23 nCPM

Immune cell

  • MAIT T-cell: 4.4 nTPM
  • T-reg: 4.3 nTPM
  • NK-cell: 4.2 nTPM
  • naive CD8 T-cell: 4.1 nTPM
  • memory CD4 T-cell: 3.8 nTPM
  • memory CD8 T-cell: 3.8 nTPM

Brain region

  • white matter: 7.7 nTPM
  • cerebellum: 7 nTPM
  • choroid plexus: 6.8 nTPM
  • basal ganglia: 6.6 nTPM
  • medulla oblongata: 6.4 nTPM
  • thalamus: 6.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.95
gnomAD pLI
0
gnomAD missense Z
0.01
DepMap mean gene effect
-0.03
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ZCCHC4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZCCHC4 as an antibody target. Whether an autoantibody or antibody against ZCCHC4 could matter depends on whether native ZCCHC4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZCCHC4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZCCHC4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ZCCHC4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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