Seroatlas · Human Serome Atlas

RAD1

Cell cycle checkpoint protein RAD1

Also known as: HRAD1, RAD1_HUMAN, REC1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O60671
Gene
RAD1
Ensembl
ENSG00000113456
Chromosome
5
Canonical length
282 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Vesicles

OverviewNCBI Gene

This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jan 2009]

Canonical amino-acid sequenceUniProt

282 residues, UniProt reviewed canonical sequence.

>O60671|RAD1
     1  MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ
    61  ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM
   121  LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS EGLREAFSEL DMTSEVLQIT
   181  MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK
   241  VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RAD1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.26
Highest tissue expression
8.6 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 8.6 nTPM
  • tongue: 7.5 nTPM
  • heart muscle: 7.4 nTPM
  • lymph node: 7.4 nTPM
  • retina: 7.3 nTPM
  • adrenal gland: 7.1 nTPM

Single-cell type

  • oocytes: 120 nCPM
  • syncytiotrophoblasts: 103 nCPM
  • cytotrophoblasts: 97 nCPM
  • migrating cytotrophoblasts: 92 nCPM
  • early primary spermatocytes: 74 nCPM
  • extravillous trophoblasts: 64 nCPM

Immune cell

  • basophil: 7.4 nTPM
  • naive CD8 T-cell: 4.9 nTPM
  • naive CD4 T-cell: 4.8 nTPM
  • eosinophil: 4.7 nTPM
  • naive B-cell: 4.7 nTPM
  • MAIT T-cell: 4.5 nTPM

Brain region

  • choroid plexus: 15 nTPM
  • cerebellum: 13 nTPM
  • hypothalamus: 13 nTPM
  • cerebral cortex: 12 nTPM
  • pons: 11 nTPM
  • basal ganglia: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.1
gnomAD pLI
0
gnomAD missense Z
0.27
DepMap mean gene effect
-0.65
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 15% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RAD1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RAD1 as an antibody target. Whether an autoantibody or antibody against RAD1 could matter depends on whether native RAD1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RAD1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RAD1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RAD1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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