Seroatlas · Human Serome Atlas

HUS1

Checkpoint protein HUS1

Also known as: HUS1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O60921
Gene
HUS1
Ensembl
ENSG00000136273
Chromosome
7
Canonical length
280 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]

Canonical amino-acid sequenceUniProt

280 residues, UniProt reviewed canonical sequence.

>O60921|HUS1
     1  MKFRAKIVDG ACLNHFTRIS NMIAKLAKTC TLRISPDKLN FILCDKLANG GVSMWCELEQ
    61  ENFFNEFQME GVSAENNEIY LELTSENLSR ALKTAQNARA LKIKLTNKHF PCLTVSVELL
   121  SMSSSSRIVT HDIPIKVIPR KLWKDLQEPV VPDPDVSIYL PVLKTMKSVV EKMKNISNHL
   181  VIEANLDGEL NLKIETELVC VTTHFKDLGN PPLASESTHE DRNVEHMAEV HIDIRKLLQF
   241  LAGQQVNPTK ALCNIVNNKM VHFDLLHEDV SLQYFIPALS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HUS1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.27
Highest tissue expression
11 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 11 nTPM
  • choroid plexus: 10 nTPM
  • liver: 10 nTPM
  • parathyroid gland: 9.1 nTPM
  • rectum: 8.1 nTPM
  • esophagus: 8 nTPM

Single-cell type

  • late spermatids: 75 nCPM
  • esophageal apical cells: 68 nCPM
  • esophageal suprabasal cells: 49 nCPM
  • monocytes: 43 nCPM
  • extravillous trophoblasts: 40 nCPM
  • syncytiotrophoblasts: 37 nCPM

Immune cell

  • basophil: 19 nTPM
  • NK-cell: 15 nTPM
  • plasmacytoid DC: 15 nTPM
  • classical monocyte: 14 nTPM
  • T-reg: 14 nTPM
  • memory CD8 T-cell: 14 nTPM

Brain region

  • choroid plexus: 16 nTPM
  • midbrain: 15 nTPM
  • thalamus: 15 nTPM
  • cerebellum: 14 nTPM
  • medulla oblongata: 14 nTPM
  • white matter: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.12
gnomAD pLI
0
gnomAD missense Z
-0.41
DepMap mean gene effect
-0.44
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 13% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of HUS1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads HUS1 as an antibody target. Whether an autoantibody or antibody against HUS1 could matter depends on whether native HUS1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

HUS1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label HUS1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/HUS1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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