Seroatlas · Human Serome Atlas

RAD17

Cell cycle checkpoint protein RAD17

Also known as: CCYC, RAD17_HUMAN, RAD17Sp, Rad24

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O75943
Gene: RAD17
Ensembl: ENSG00000152942
Chromosome: 5
Canonical length: 681 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013]

Canonical amino-acid sequenceUniProt

681 residues, UniProt reviewed canonical sequence.

>O75943|RAD17
     1  MSKTFLRPKV SSTKVTDWVD PSFDDFLECS GVSTITATSL GVNNSSHRRK NGPSTLESSR
    61  FPARKRGNLS SLEQIYGLEN SKEYLSENEP WVDKYKPETQ HELAVHKKKI EEVETWLKAQ
   121  VLERQPKQGG SILLITGPPG CGKTTTLKIL SKEHGIQVQE WINPVLPDFQ KDDFKGMFNT
   181  ESSFHMFPYQ SQIAVFKEFL LRATKYNKLQ MLGDDLRTDK KIILVEDLPN QFYRDSHTLH
   241  EVLRKYVRIG RCPLIFIISD SLSGDNNQRL LFPKEIQEEC SISNISFNPV APTIMMKFLN
   301  RIVTIEANKN GGKITVPDKT SLELLCQGCS GDIRSAINSL QFSSSKGENN LRPRKKGMSL
   361  KSDAVLSKSK RRKKPDRVFE NQEVQAIGGK DVSLFLFRAL GKILYCKRAS LTELDSPRLP
   421  SHLSEYERDT LLVEPEEVVE MSHMPGDLFN LYLHQNYIDF FMEIDDIVRA SEFLSFADIL
   481  SGDWNTRSLL REYSTSIATR GVMHSNKARG YAHCQGGGSS FRPLHKPQWF LINKKYRENC
   541  LAAKALFPDF CLPALCLQTQ LLPYLALLTI PMRNQAQISF IQDIGRLPLK RHFGRLKMEA
   601  LTDREHGMID PDSGDEAQLN GGHSAEESLG EPTQATVPET WSLPLSQNSA SELPASQPQP
   661  FSAQGDMEEN IIIEDYESDG T

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RAD17 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.44
Highest tissue expression: 39 nTPM

Expression across tissuesHPA

Tissue

testis: 39 nTPM
lymph node: 18 nTPM
pancreas: 16 nTPM
pituitary gland: 15 nTPM
retina: 15 nTPM
kidney: 14 nTPM

Single-cell type

early primary spermatocytes: 57 nCPM
late primary spermatocytes: 52 nCPM
distal convoluted tubule cells: 45 nCPM
early spermatids: 42 nCPM
renal collecting duct intercalated cells: 39 nCPM
loop of henle epithelial cells: 38 nCPM

Immune cell

memory B-cell: 7.5 nTPM
NK-cell: 6.5 nTPM
T-reg: 5.8 nTPM
naive B-cell: 5.5 nTPM
naive CD4 T-cell: 5.4 nTPM
naive CD8 T-cell: 4.7 nTPM

Brain region

hypothalamus: 9.2 nTPM
white matter: 9 nTPM
midbrain: 8.2 nTPM
choroid plexus: 8.1 nTPM
cerebellum: 8 nTPM
cerebral cortex: 7.7 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.45
gnomAD pLI: 0.06
gnomAD missense Z: 0.62
DepMap mean gene effect: -1.05
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

P-loop containing nucleoside triphosphate hydrolase
Checkpoint protein Rad17/Rad24
Checkpoint protein Rad17/Rad24, fungi/metazoa
Rad17 P-loop domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RAD17 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RAD17 as an antibody target. Whether an autoantibody or antibody against RAD17 could matter depends on whether native RAD17 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RAD17 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RAD17 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...