Seroatlas · Human Serome Atlas

RACGAP1

Rac GTPase-activating protein 1

Also known as: CYK4, MgcRacGAP, RGAP1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9H0H5
Gene
RACGAP1
Ensembl
ENSG00000161800
Chromosome
12
Canonical length
632 aa
Protein class
Disease related genes, Human disease related genes, Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]

Canonical amino-acid sequenceUniProt

632 residues, UniProt reviewed canonical sequence.

>Q9H0H5|RACGAP1
     1  MDTMMLNVRN LFEQLVRRVE ILSEGNEVQF IQLAKDFEDF RKKWQRTDHE LGKYKDLLMK
    61  AETERSALDV KLKHARNQVD VEIKRRQRAE ADCEKLERQI QLIREMLMCD TSGSIQLSEE
   121  QKSALAFLNR GQPSSSNAGN KRLSTIDESG SILSDISFDK TDESLDWDSS LVKTFKLKKR
   181  EKRRSTSRQF VDGPPGPVKK TRSIGSAVDQ GNESIVAKTT VTVPNDGGPI EAVSTIETVP
   241  YWTRSRRKTG TLQPWNSDST LNSRQLEPRT ETDSVGTPQS NGGMRLHDFV SKTVIKPESC
   301  VPCGKRIKFG KLSLKCRDCR VVSHPECRDR CPLPCIPTLI GTPVKIGEGM LADFVSQTSP
   361  MIPSIVVHCV NEIEQRGLTE TGLYRISGCD RTVKELKEKF LRVKTVPLLS KVDDIHAICS
   421  LLKDFLRNLK EPLLTFRLNR AFMEAAEITD EDNSIAAMYQ AVGELPQANR DTLAFLMIHL
   481  QRVAQSPHTK MDVANLAKVF GPTIVAHAVP NPDPVTMLQD IKRQPKVVER LLSLPLEYWS
   541  QFMMVEQENI DPLHVIENSN AFSTPQTPDI KVSLLGPVTT PEHQLLKTPS SSSLSQRVRS
   601  TLTKNTPRFG SKSKSATNLG RQGNFFASPM LK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RACGAP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.47
Highest tissue expression
43 nTPM

Expression across tissuesHPA

Tissue

  • testis: 43 nTPM
  • thymus: 40 nTPM
  • bone marrow: 37 nTPM
  • tonsil: 28 nTPM
  • lymph node: 22 nTPM
  • esophagus: 20 nTPM

Single-cell type

  • monocyte progenitors: 106 nCPM
  • early primary spermatocytes: 90 nCPM
  • erythrocyte progenitors: 76 nCPM
  • neutrophil progenitors: 66 nCPM
  • extravillous trophoblasts: 63 nCPM
  • late primary spermatocytes: 53 nCPM

Immune cell

  • basophil: 24 nTPM
  • T-reg: 19 nTPM
  • NK-cell: 11 nTPM
  • memory CD8 T-cell: 9.2 nTPM
  • memory B-cell: 8.3 nTPM
  • memory CD4 T-cell: 7.9 nTPM

Brain region

  • basal ganglia: 17 nTPM
  • thalamus: 17 nTPM
  • midbrain: 15 nTPM
  • cerebellum: 15 nTPM
  • white matter: 15 nTPM
  • medulla oblongata: 14 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RACGAP1.

Disease | AllUniProt

Conditions RACGAP1 is implicated in, by any mechanism.

  • Anemia, congenital dyserythropoietic, 3B, autosomal recessive (CDAN3B) MIM:619789

Disease | GeneticClinVar

3 pathogenic / likely-pathogenic of 96 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Anemia, congenital dyserythropoietic, type IIIb
  • Anemia, congenital dyserythropoietic, type IIIb, autosomal recessive

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.52
gnomAD pLI
0
gnomAD missense Z
2.01
DepMap mean gene effect
-1.48
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RACGAP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RACGAP1 as an antibody target. Whether an autoantibody or antibody against RACGAP1 could matter depends on whether native RACGAP1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RACGAP1 is annotated at the cell surface, where native RACGAP1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label RACGAP1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RACGAP1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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