Seroatlas · Human Serome Atlas

PIAS3

E3 SUMO-protein ligase PIAS3

Also known as: FLJ14651, PIAS3_HUMAN, ZMIZ5

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y6X2
Gene: PIAS3
Ensembl: ENSG00000131788
Chromosome: 1
Canonical length: 628 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

628 residues, UniProt reviewed canonical sequence.

>Q9Y6X2|PIAS3
     1  MAELGELKHM VMSFRVSELQ VLLGFAGRNK SGRKHELLAK ALHLLKSSCA PSVQMKIKEL
    61  YRRRFPRKTL GPSDLSLLSL PPGTSPVGSP GPLAPIPPTL LAPGTLLGPK REVDMHPPLP
   121  QPVHPDVTMK PLPFYEVYGE LIRPTTLAST SSQRFEEAHF TFALTPQQVQ QILTSREVLP
   181  GAKCDYTIQV QLRFCLCETS CPQEDYFPPN LFVKVNGKLC PLPGYLPPTK NGAEPKRPSR
   241  PINITPLARL SATVPNTIVV NWSSEFGRNY SLSVYLVRQL TAGTLLQKLR AKGIRNPDHS
   301  RALIKEKLTA DPDSEVATTS LRVSLMCPLG KMRLTVPCRA LTCAHLQSFD AALYLQMNEK
   361  KPTWTCPVCD KKAPYESLII DGLFMEILSS CSDCDEIQFM EDGSWCPMKP KKEASEVCPP
   421  PGYGLDGLQY SPVQGGDPSE NKKKVEVIDL TIESSSDEED LPPTKKHCSV TSAAIPALPG
   481  SKGVLTSGHQ PSSVLRSPAM GTLGGDFLSS LPLHEYPPAF PLGADIQGLD LFSFLQTESQ
   541  HYGPSVITSL DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP GRVSSIVAPG
   601  GALREGHGGP LPSGPSLTGC RSDIISLD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PIAS3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.49
Highest tissue expression: 37 nTPM

Expression across tissuesHPA

Tissue

blood vessel: 37 nTPM
fallopian tube: 35 nTPM
esophagus: 35 nTPM
skin: 32 nTPM
endometrium: 32 nTPM
vagina: 32 nTPM

Single-cell type

esophageal suprabasal cells: 51 nCPM
fallopian tube ciliated cells: 51 nCPM
esophageal apical cells: 41 nCPM
respiratory ciliated cells: 38 nCPM
endometrial ciliated cells: 36 nCPM
retinal pigment epithelial cells: 30 nCPM

Immune cell

NK-cell: 15 nTPM
non-classical monocyte: 13 nTPM
memory CD8 T-cell: 12 nTPM
MAIT T-cell: 11 nTPM
eosinophil: 11 nTPM
gdT-cell: 11 nTPM

Brain region

hypothalamus: 26 nTPM
choroid plexus: 25 nTPM
cerebral cortex: 25 nTPM
midbrain: 23 nTPM
thalamus: 22 nTPM
medulla oblongata: 22 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about PIAS3.

Disease | ImmuneIEDB

Conditions an epitope on PIAS3 was assayed in.

breast cancer T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.3
gnomAD pLI: 0.98
gnomAD missense Z: 1
DepMap mean gene effect: -0.19
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PIAS3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PIAS3 as an antibody target. Whether an autoantibody or antibody against PIAS3 could matter depends on whether native PIAS3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PIAS3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PIAS3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...