PFDN5
Prefoldin subunit 5
Also known as: MM-1, PFD5, PFD5_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q99471
- Gene
- PFDN5
- Ensembl
- ENSG00000123349
- Chromosome
- 12
- Canonical length
- 154 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Intermediate filaments,Cytosol
OverviewNCBI Gene
This gene encodes a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
154 residues, UniProt reviewed canonical sequence.
>Q99471|PFDN5
1 MAQSINITEL NLPQLEMLKN QLDQEVEFLS TSIAQLKVVQ TKYVEAKDCL NVLNKSNEGK
61 ELLVPLTSSM YVPGKLHDVE HVLIDVGTGY YVEKTAEDAK DFFKRKIDFL TKQMEKIQPA
121 LQEKHAMKQA VMEMMSQKIQ QLTALGAAQA TAKALocalizationUniProt · AlphaFold · HPA
Whether an antibody against PFDN5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.39
- Highest tissue expression
- 1,060 nTPM
Expression across tissuesHPA
Tissue
- choroid plexus: 1,060 nTPM
- ovary: 964 nTPM
- spinal cord: 915 nTPM
- amygdala: 898 nTPM
- midbrain: 898 nTPM
- epididymis: 818 nTPM
Single-cell type
- papillary tip epithelial cells: 62 nCPM
- renal collecting duct principal cells: 53 nCPM
- brain excitatory neurons: 51 nCPM
- oligodendrocytes: 49 nCPM
- other brain neurons: 49 nCPM
- astrocytes: 49 nCPM
Immune cell
- total PBMC: 8,669 nTPM
- memory B-cell: 3,723 nTPM
- naive B-cell: 3,385 nTPM
- naive CD4 T-cell: 3,158 nTPM
- classical monocyte: 2,771 nTPM
- naive CD8 T-cell: 2,541 nTPM
Brain region
- spinal cord: 301 nTPM
- thalamus: 284 nTPM
- white matter: 272 nTPM
- basal ganglia: 254 nTPM
- medulla oblongata: 241 nTPM
- hypothalamus: 239 nTPM
ReferencesPubMed · IEDB
Publications for PFDN5 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.
Reference: AutoantibodyPubMed
2 publications
- Prefoldin 5 and Anti-prefoldin 5 Antibodies as Biomarkers for Uveitis in Ankylosing Spondylitis.
2019 · Front Immunol · RCR 0.7 · 16 citations - Serological biomarkers of JIA-associated uveitis: ANA titers, ANA AC-30 staining pattern and prefoldin 5 antibodies.
2026 · Arthritis Res Ther
Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.33
- gnomAD pLI
- 0
- gnomAD missense Z
- 0.1
- DepMap mean gene effect
- -0.78
- DepMap dependency class
- common
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- negative regulation of amyloid fibril formation
- negative regulation of canonical Wnt signaling pathway
- negative regulation of DNA-templated transcription
- protein folding
- regulation of DNA-templated transcription
- retina development in camera-type eye
- RNA polymerase II core complex assembly
- RNA polymerase I assembly
- RNA polymerase III assembly
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Prefoldin alpha-like
- Prefoldin
- Prefoldin subunit
- Prefoldin alpha subunit, archaea-type
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of PFDN5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads PFDN5 as an antibody target. Whether an autoantibody or antibody against PFDN5 could matter depends on whether native PFDN5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
PFDN5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label PFDN5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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