Seroatlas · Human Serome Atlas

RNF20

E3 ubiquitin-protein ligase BRE1A

Also known as: BRE1, BRE1A, BRE1A_HUMAN, FLJ11189, FLJ20382, hBRE1, KAIA2779

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5VTR2
Gene: RNF20
Ensembl: ENSG00000155827
Chromosome: 9
Canonical length: 975 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). [provided by RefSeq, Feb 2012]

Canonical amino-acid sequenceUniProt

975 residues, UniProt reviewed canonical sequence.

>Q5VTR2|RNF20
     1  MSGIGNKRAA GEPGTSMPPE KKAAVEDSGT TVETIKLGGV SSTEELDIRT LQTKNRKLAE
    61  MLDQRQAIED ELREHIEKLE RRQATDDASL LIVNRYWSQF DENIRIILKR YDLEQGLGDL
   121  LTERKALVVP EPEPDSDSNQ ERKDDRERGE GQEPAFSFLA TLASSSSEEM ESQLQERVES
   181  SRRAVSQIVT VYDKLQEKVE LLSRKLNSGD NLIVEEAVQE LNSFLAQENM RLQELTDLLQ
   241  EKHRTMSQEF SKLQSKVETA ESRVSVLESM IDDLQWDIDK IRKREQRLNR HLAEVLERVN
   301  SKGYKVYGAG SSLYGGTITI NARKFEEMNA ELEENKELAQ NRLCELEKLR QDFEEVTTQN
   361  EKLKVELRSA VEQVVKETPE YRCMQSQFSV LYNESLQLKA HLDEARTLLH GTRGTHQHQV
   421  ELIERDEVSL HKKLRTEVIQ LEDTLAQVRK EYEMLRIEFE QTLAANEQAG PINREMRHLI
   481  SSLQNHNHQL KGEVLRYKRK LREAQSDLNK TRLRSGSALL QSQSSTEDPK DEPAELKPDS
   541  EDLSSQSSAS KASQEDANEI KSKRDEEERE RERREKERER EREREKEKER EREKQKLKES
   601  EKERDSAKDK EKGKHDDGRK KEAEIIKQLK IELKKAQESQ KEMKLLLDMY RSAPKEQRDK
   661  VQLMAAEKKS KAELEDLRQR LKDLEDKEKK ENKKMADEDA LRKIRAVEEQ IEYLQKKLAM
   721  AKQEEEALLS EMDVTGQAFE DMQEQNIRLM QQLREKDDAN FKLMSERIKS NQIHKLLKEE
   781  KEELADQVLT LKTQVDAQLQ VVRKLEEKEH LLQSNIGTGE KELGLRTQAL EMNKRKAMEA
   841  AQLADDLKAQ LELAQKKLHD FQDEIVENSV TKEKDMFNFK RAQEDISRLR RKLETTKKPD
   901  NVPKCDEILM EEIKDYKARL TCPCCNMRKK DAVLTKCFHV FCFECVKTRY DTRQRKCPKC
   961  NAAFGANDFH RIYIG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF20 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.49
Highest tissue expression: 30 nTPM

Expression across tissuesHPA

Tissue

testis: 30 nTPM
retina: 28 nTPM
skeletal muscle: 28 nTPM
parathyroid gland: 26 nTPM
heart muscle: 25 nTPM
cerebral cortex: 24 nTPM

Single-cell type

late primary spermatocytes: 155 nCPM
esophageal apical cells: 62 nCPM
prostatic glandular cells: 51 nCPM
corticotrophs: 46 nCPM
neutrophils: 44 nCPM
neutrophil progenitors: 41 nCPM

Immune cell

non-classical monocyte: 30 nTPM
basophil: 25 nTPM
eosinophil: 24 nTPM
gdT-cell: 21 nTPM
intermediate monocyte: 20 nTPM
naive CD4 T-cell: 17 nTPM

Brain region

white matter: 43 nTPM
basal ganglia: 40 nTPM
midbrain: 39 nTPM
medulla oblongata: 38 nTPM
hypothalamus: 38 nTPM
cerebral cortex: 36 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.62
gnomAD pLI: 0
gnomAD missense Z: 3.11
DepMap mean gene effect: -0.81
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

chromatin binding
histone binding
identical protein binding
mRNA 3'-UTR binding
p53 binding
transcription coactivator activity
ubiquitin protein ligase activity
ubiquitin protein ligase binding
ubiquitin-protein transferase activity
zinc ion binding
histone H2B C-terminal K residue ubiquitin ligase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RNF20 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF20 as an antibody target. Whether an autoantibody or antibody against RNF20 could matter depends on whether native RNF20 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF20 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF20 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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