Seroatlas · Human Serome Atlas

RNF40

E3 ubiquitin-protein ligase BRE1B

Also known as: BRE1B, BRE1B_HUMAN, KIAA0661, RBP95, STARING

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O75150
Gene
RNF40
Ensembl
ENSG00000103549
Chromosome
16
Canonical length
1001 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

Canonical amino-acid sequenceUniProt

1001 residues, UniProt reviewed canonical sequence.

>O75150|RNF40
     1  MSGPGNKRAA GDGGSGPPEK KLSREEKTTT TLIEPIRLGG ISSTEEMDLK VLQFKNKKLA
    61  ERLEQRQACE DELRERIEKL EKRQATDDAT LLIVNRYWAQ LDETVEALLR CHESQGELSS
   121  APEAPGTQEG PTCDGTPLPE PGTSELRDPL LMQLRPPLSE PALAFVVALG ASSSEEVELE
   181  LQGRMEFSKA AVSRVVEASD RLQRRVEELC QRVYSRGDSE PLSEAAQAHT RELGRENRRL
   241  QDLATQLQEK HHRISLEYSE LQDKVTSAET KVLEMETTVE DLQWDIEKLR KREQKLNKHL
   301  AEALEQLNSG YYVSGSSSGF QGGQITLSMQ KFEMLNAELE ENQELANSRM AELEKLQAEL
   361  QGAVRTNERL KVALRSLPEE VVRETGEYRM LQAQFSLLYN ESLQVKTQLD EARGLLLATK
   421  NSHLRHIEHM ESDELGLQKK LRTEVIQLED TLAQVRKEYE MLRIEFEQNL AANEQAGPIN
   481  REMRHLISSL QNHNHQLKGD AQRYKRKLRE VQAEIGKLRA QASGSAHSTP NLGHPEDSGV
   541  SAPAPGKEEG GPGPVSTPDN RKEMAPVPGT TTTTTSVKKE ELVPSEEDFQ GITPGAQGPS
   601  SRGREPEARP KRELREREGP SLGPPPVASA LSRADREKAK VEETKRKESE LLKGLRAELK
   661  KAQESQKEMK LLLDMYKSAP KEQRDKVQLM AAERKAKAEV DELRSRIREL EERDRRESKK
   721  IADEDALRRI RQAEEQIEHL QRKLGATKQE EEALLSEMDV TGQAFEDMQE QNGRLLQQLR
   781  EKDDANFKLM SERIKANQIH KLLREEKDEL GEQVLGLKSQ VDAQLLTVQK LEEKERALQG
   841  SLGGVEKELT LRSQALELNK RKAVEAAQLA EDLKVQLEHV QTRLREIQPC LAESRAAREK
   901  ESFNLKRAQE DISRLRRKLE KQRKVEVYAD ADEILQEEIK EYKARLTCPC CNTRKKDAVL
   961  TKCFHVFCFE CVRGRYEARQ RKCPKCNAAF GAHDFHRIYI S

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF40 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.52
Highest tissue expression
52 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 52 nTPM
  • testis: 36 nTPM
  • parathyroid gland: 33 nTPM
  • salivary gland: 32 nTPM
  • cerebellum: 30 nTPM
  • esophagus: 30 nTPM

Single-cell type

  • late primary spermatocytes: 57 nCPM
  • early primary spermatocytes: 51 nCPM
  • sertoli cells: 51 nCPM
  • cholangiocytes: 49 nCPM
  • endometrial luminal cells: 46 nCPM
  • esophageal apical cells: 46 nCPM

Immune cell

  • eosinophil: 17 nTPM
  • non-classical monocyte: 17 nTPM
  • total PBMC: 16 nTPM
  • neutrophil: 16 nTPM
  • intermediate monocyte: 14 nTPM
  • myeloid DC: 14 nTPM

Brain region

  • white matter: 58 nTPM
  • thalamus: 56 nTPM
  • choroid plexus: 54 nTPM
  • medulla oblongata: 54 nTPM
  • hippocampal formation: 53 nTPM
  • basal ganglia: 53 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.24
gnomAD pLI
1
gnomAD missense Z
2.7
DepMap mean gene effect
-0.37
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RNF40 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF40 as an antibody target. Whether an autoantibody or antibody against RNF40 could matter depends on whether native RNF40 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF40 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF40 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RNF40. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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