NOP53
Ribosome biogenesis protein NOP53
Also known as: GLTSCR2, NOP53_HUMAN, PICT-1, PICT1
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q9NZM5
- Gene
- NOP53
- Ensembl
- ENSG00000105373
- Chromosome
- 19
- Canonical length
- 478 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Nucleoli
- Quaternary structure
- Homooligomer
OverviewNCBI Gene
Enables 5S rRNA binding activity; identical protein binding activity; and p53 binding activity. Involved in several processes, including mitotic G2 DNA damage checkpoint signaling; regulation of intracellular signal transduction; and regulation of primary metabolic process. Located in cytosol; nuclear lumen; and rDNA heterochromatin. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
478 residues, UniProt reviewed canonical sequence.
>Q9NZM5|NOP53
1 MAAGGSGVGG KRSSKSDADS GFLGLRPTSV DPALRRRRRG PRNKKRGWRR LAQEPLGLEV
61 DQFLEDVRLQ ERTSGGLLSE APNEKLFFVD TGSKEKGLTK KRTKVQKKSL LLKKPLRVDL
121 ILENTSKVPA PKDVLAHQVP NAKKLRRKEQ LWEKLAKQGE LPREVRRAQA RLLNPSATRA
181 KPGPQDTVER PFYDLWASDN PLDRPLVGQD EFFLEQTKKK GVKRPARLHT KPSQAPAVEV
241 APAGASYNPS FEDHQTLLSA AHEVELQRQK EAEKLERQLA LPATEQAATQ ESTFQELCEG
301 LLEESDGEGE PGQGEGPEAG DAEVCPTPAR LATTEKKTEQ QRRREKAVHR LRVQQAALRA
361 ARLRHQELFR LRGIKAQVAL RLAELARRQR RRQARREAEA DKPRRLGRLK YQAPDIDVQL
421 SSELTDSLRT LKPEGNILRD RFKSFQRRNM IEPRERAKFK RKYKVKLVEK RAFREIQLLocalizationUniProt · AlphaFold · HPA
Whether an antibody against NOP53 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.54
- Highest tissue expression
- 1,016 nTPM
Expression across tissuesHPA
Tissue
- pancreas: 1,016 nTPM
- ovary: 793 nTPM
- skin: 628 nTPM
- cervix: 514 nTPM
- urinary bladder: 454 nTPM
- colon: 434 nTPM
Single-cell type
- pancreatic acinar cells: 1,101 nCPM
- ovarian stromal cells: 963 nCPM
- breast secretory cells: 881 nCPM
- decidual stromal cells: 794 nCPM
- enteric stem cells: 767 nCPM
- extravillous trophoblasts: 735 nCPM
Immune cell
- memory B-cell: 314 nTPM
- naive CD4 T-cell: 246 nTPM
- naive B-cell: 241 nTPM
- T-reg: 120 nTPM
- naive CD8 T-cell: 109 nTPM
- memory CD4 T-cell: 109 nTPM
Brain region
- medulla oblongata: 109 nTPM
- spinal cord: 97 nTPM
- thalamus: 95 nTPM
- choroid plexus: 91 nTPM
- white matter: 87 nTPM
- basal ganglia: 87 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.49
- gnomAD pLI
- 0.18
- DepMap mean gene effect
- -0.56
- DepMap dependency class
- common
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cellular response to hypoxia
- DNA damage response
- DNA repair
- mitotic G2 DNA damage checkpoint signaling
- negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
- negative regulation of proteasomal ubiquitin-dependent protein catabolic process
- negative regulation of protein-containing complex assembly
- negative regulation of signal transduction by p53 class mediator
- negative regulation of transcription by RNA polymerase II
- negative regulation of transcription of nucleolar large rRNA by RNA polymerase I
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process
- positive regulation of protein K63-linked deubiquitination
- protein localization to nucleolus
- protein localization to nucleoplasm
- protein stabilization
- regulation of aerobic respiration
- regulation of apoptotic process
- regulation of cell cycle
- regulation of protein phosphorylation
- regulation of RIG-I signaling pathway
- regulation of signal transduction by p53 class mediator
- ribosomal large subunit assembly
- rRNA processing
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Ribosome biogenesis protein Nop53/GLTSCR2
- Nop53 (60S ribosomal biogenesis)
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of NOP53 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads NOP53 as an antibody target. Whether an autoantibody or antibody against NOP53 could matter depends on whether native NOP53 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
NOP53 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label NOP53 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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