NFAT5

Protein identityUniProt · HPA

UniProt accession

O94916

Gene

NFAT5

Ensembl

ENSG00000102908

Chromosome

16

Canonical length

1531 aa

Protein class

Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm,Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1531 residues, UniProt reviewed canonical sequence.

>O94916|NFAT5
     1  MPSDFISLLS ADLDLESPKS LYSRESVYDL LPKELQLPPS RETSVASMSQ TSGGEAGSPP
    61  PAVVAADASS APSSSSMGGA CSSFTTSSSP TIYSTSVTDS KAMQVESCSS AVGVSNRGVS
   121  EKQLTSNTVQ QHPSTPKRHT VLYISPPPED LLDNSRMSCQ DEGCGLESEQ SCSMWMEDSP
   181  SNFSNMSTSS YNDNTEVPRK SRKRNPKQRP GVKRRDCEES NMDIFDADSA KAPHYVLSQL
   241  TTDNKGNSKA GNGTLENQKG TGVKKSPMLC GQYPVKSEGK ELKIVVQPET QHRARYLTEG
   301  SRGSVKDRTQ QGFPTVKLEG HNEPVVLQVF VGNDSGRVKP HGFYQACRVT GRNTTPCKEV
   361  DIEGTTVIEV GLDPSNNMTL AVDCVGILKL RNADVEARIG IAGSKKKSTR ARLVFRVNIM
   421  RKDGSTLTLQ TPSSPILCTQ PAGVPEILKK SLHSCSVKGE EEVFLIGKNF LKGTKVIFQE
   481  NVSDENSWKS EAEIDMELFH QNHLIVKVPP YHDQHITLPV SVGIYVVTNA GRSHDVQPFT
   541  YTPDPAAAGA LNVNVKKEIS SPARPCSFEE AMKAMKTTGC NLDKVNIIPN ALMTPLIPSS
   601  MIKSEDVTPM EVTAEKRSST IFKTTKSVGS TQQTLENISN IAGNGSFSSP SSSHLPSENE
   661  KQQQIQPKAY NPETLTTIQT QDISQPGTFP AVSASSQLPN SDALLQQATQ FQTRETQSRE
   721  ILQSDGTVVN LSQLTEASQQ QQQSPLQEQA QTLQQQISSN IFPSPNSVSQ LQNTIQQLQA
   781  GSFTGSTASG SSGSVDLVQQ VLEAQQQLSS VLFSAPDGNE NVQEQLSADI FQQVSQIQSG
   841  VSPGMFSSTE PTVHTRPDNL LPGRAESVHP QSENTLSNQQ QQQQQQQQVM ESSAAMVMEM
   901  QQSICQAAAQ IQSELFPSTA SANGNLQQSP VYQQTSHMMS ALSTNEDMQM QCELFSSPPA
   961  VSGNETSTTT TQQVATPGTT MFQTSSSGDG EETGTQAKQI QNSVFQTMVQ MQHSGDNQPQ
  1021  VNLFSSTKSM MSVQNSGTQQ QGNGLFQQGN EMMSLQSGNF LQQSSHSQAQ LFHPQNPIAD
  1081  AQNLSQETQG SLFHSPNPIV HSQTSTTSSE QMQPPMFHSQ STIAVLQGSS VPQDQQSTNI
  1141  FLSQSPMNNL QTNTVAQEAF FAAPNSISPL QSTSNSEQQA AFQQQAPISH IQTPMLSQEQ
  1201  AQPPQQGLFQ PQVALGSLPP NPMPQSQQGT MFQSQHSIVA MQSNSPSQEQ QQQQQQQQQQ
  1261  QQQQQQSILF SNQNTMATMA SPKQPPPNMI FNPNQNPMAN QEQQNQSIFH QQSNMAPMNQ
  1321  EQQPMQFQSQ STVSSLQNPG PTQSESSQTP LFHSSPQIQL VQGSPSSQEQ QVTLFLSPAS
  1381  MSALQTSINQ QDMQQSPLYS PQNNMPGIQG ATSSPQPQAT LFHNTAGGTM NQLQNSPGSS
  1441  QQTSGMFLFG IQNNCSQLLT SGPATLPDQL MAISQPGQPQ NEGQPPVTTL LSQQMPENSP
  1501  LASSINTNQN IEKIDLLVSL QNQGNNLTGS F

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NFAT5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.61

Highest tissue expression

5.3 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.21

gnomAD pLI

1

gnomAD missense Z

3.13

DepMap mean gene effect

-0.13

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• calcineurin-NFAT signaling cascade
• cellular hyperosmotic response
• cellular response to cytokine stimulus
• DNA damage response
• positive regulation of canonical NF-kappaB signal transduction
• positive regulation of gene expression
• positive regulation of leukocyte adhesion to vascular endothelial cell
• positive regulation of transcription by RNA polymerase II
• R-loop processing
• regulation of calcineurin-NFAT signaling cascade
• signal transduction
• transcription by RNA polymerase II

Molecular functions

• chromatin binding
• DNA-binding transcription activator activity, RNA polymerase II-specific
• DNA-binding transcription factor activity
• DNA-binding transcription factor activity, RNA polymerase II-specific
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• sequence-specific double-stranded DNA binding

Cellular components

• chromatin
• cytosol
• nucleoplasm
• nucleus
• transcription regulator complex

Protein domainsUniProt · Pfam · InterPro

• IPT domain
• Nuclear factor of activated T cells (NFAT)
• p53-like transcription factor, DNA-binding domain superfamily
• Rel homology domain, DNA-binding domain
• Immunoglobulin-like fold
• Immunoglobulin E-set
• Rel homology dimerisation domain
• Rel homology domain (RHD), DNA-binding domain superfamily
• Rel homology DNA-binding domain
• Rel homology dimerisation domain
• Nuclear factor of activated T-cells 5, Rel homology domain, DNA-binding domain

KeywordsUniProt

• Acetylation
• Activator
• ADP-ribosylation
• Chromosome
• Cytoplasm
• DNA damage
• DNA-binding
• Isopeptide bond
• Nucleus
• Phosphoprotein
• Transcription
• Transcription regulation
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of NFAT5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CIDEC
• DDX17
• DDX5
• NFE2L2