CENPC

Protein identityUniProt · HPA

UniProt accession

Q03188

Gene

CENPC

Ensembl

ENSG00000145241

Chromosome

4

Canonical length

943 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nuclear bodies,Kinetochore,Midbody

OverviewNCBI Gene

Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

943 residues, UniProt reviewed canonical sequence.

>Q03188|CENPC
     1  MAASGLDHLK NGYRRRFCRP SRARDINTEQ GQNVLEILQD CFEEKSLAND FSTNSTKSVP
    61  NSTRKIKDTC IQSPSKECQK SHPKSVPVSS KKKEASLQFV VEPSEATNRS VQAHEVHQKI
   121  LATDVSSKNT PDSKKISSRN INDHHSEADE EFYLSVGSPS VLLDAKTSVS QNVIPSSAQK
   181  RETYTFENSV NMLPSSTEVS VKTKKRLNFD DKVMLKKIEI DNKVSDEEDK TSEGQERKPS
   241  GSSQNRIRDS EYEIQRQAKK SFSTLFLETV KRKSESSPIV RHAATAPPHS CPPDDTKLIE
   301  DEFIIDESDQ SFASRSWITI PRKAGSLKQR TISPAESTAL LQGRKSREKH HNILPKTLAN
   361  DKHSHKPHPV ETSQPSDKTV LDTSYALIGE TVNNYRSTKY EMYSKNAEKP SRSKRTIKQK
   421  QRRKFMAKPA EEQLDVGQSK DENIHTSHIT QDEFQRNSDR NMEEHEEMGN DCVSKKQMPP
   481  VGSKKSSTRK DKEESKKKRF SSESKNKLVP EEVTSTVTKS RRISRRPSDW WVVKSEESPV
   541  YSNSSVRNEL PMHHNSSRKS TKKTNQSSKN IRKKTIPLKR QKTATKGNQR VQKFLNAEGS
   601  GGIVGHDEIS RCSLSEPLES DEADLAKKKN LDCSRSTRSS KNEDNIMTAQ NVPLKPQTSG
   661  YTCNIPTESN LDSGEHKTSV LEESGPSRLN NNYLMSGKND VDDEEVHGSS DDSKQSKVIP
   721  KNRIHHKLVL PSNTPNVRRT KRTRLKPLEY WRGERIDYQG RPSGGFVISG VLSPDTISSK
   781  RKAKENIGKV NKKSNKKRIC LDNDERKTNL MVNLGIPLGD PLQPTRVKDP ETREIILMDL
   841  VRPQDTYQFF VKHGELKVYK TLDTPFFSTG KLILGPQEEK GKQHVGQDIL VFYVNFGDLL
   901  CTLHETPYIL STGDSFYVPS GNYYNIKNLR NEESVLLFTQ IKR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CENPC can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.64

Highest tissue expression

5.5 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CENPC.

Disease | ImmuneIEDB

Conditions an epitope on CENPC was assayed in.

• systemic scleroderma B cell

ReferencesPubMed · IEDB

Publications for CENPC from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.55

gnomAD pLI

0

gnomAD missense Z

0.76

DepMap mean gene effect

-0.96

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• attachment of mitotic spindle microtubules to kinetochore
• cell division
• chromosome segregation
• kinetochore assembly
• mitotic cell cycle
• spindle attachment to meiosis I kinetochore

Molecular functions

• centromeric DNA binding
• DNA binding
• identical protein binding

Cellular components

• condensed chromosome, centromeric region
• cytosol
• inner kinetochore
• kinetochore
• midbody
• nuclear body
• nucleoplasm
• nucleus
• pericentric heterochromatin

Protein domainsUniProt · Pfam · InterPro

• RmlC-like cupin domain superfamily
• RmlC-like jelly roll fold
• Mif2/CENP-C cupin domain
• Kinetochore assembly subunit CENP-C, N-terminal domain
• Centromere protein C/Mif2/cnp3
• CENP-C, middle DNMT3B-binding domain
• Mif2/CENP-C like
• Centromere assembly component CENP-C middle DNMT3B-binding region
• Kinetochore assembly subunit CENP-C N-terminal

KeywordsUniProt

• Cell cycle
• Cell division
• Centromere
• Chromosome
• DNA-binding
• Isopeptide bond
• Kinetochore
• Mitosis
• Nucleus
• Phosphoprotein
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of CENPC in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CENPA
• CENPK
• CENPL
• CENPO
• CENPP
• CENPQ
• CENPR
• CENPS
• DNMT3B
• MEIKIN
• DAXX
• H1-5
• MIS12