MAGEA4

Protein identityUniProt · HPA

UniProt accession

P43358

Gene

MAGEA4

Ensembl

ENSG00000147381

Chromosome

X

Canonical length

317 aa

Protein class

Cancer-related genes, Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Nuclear speckles,Cytosol

OverviewNCBI Gene

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2020]

Canonical amino-acid sequenceUniProt

317 residues, UniProt reviewed canonical sequence.

>P43358|MAGEA4
     1  MSSEQKSQHC KPEEGVEAQE EALGLVGAQA PTTEEQEAAV SSSSPLVPGT LEEVPAAESA
    61  GPPQSPQGAS ALPTTISFTC WRQPNEGSSS QEEEGPSTSP DAESLFREAL SNKVDELAHF
   121  LLRKYRAKEL VTKAEMLERV IKNYKRCFPV IFGKASESLK MIFGIDVKEV DPASNTYTLV
   181  TCLGLSYDGL LGNNQIFPKT GLLIIVLGTI AMEGDSASEE EIWEELGVMG VYDGREHTVY
   241  GEPRKLLTQD WVQENYLEYR QVPGSNPARY EFLWGPRALA ETSYVKVLEH VVRVNARVRI
   301  AYPSLREAAL LEEEEGV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAGEA4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.41

Highest tissue expression

21 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MAGEA4.

Disease | ImmuneIEDB

Conditions an epitope on MAGEA4 was assayed in.

• melanoma T cell
• skin melanoma T cell
• acral lentiginous melanoma T cell
• hepatocellular carcinoma T cell
• lung non-small cell carcinoma T cell
• testicular germ cell cancer T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD missense Z

-1.54

DepMap mean gene effect

0.07

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• negative regulation of apoptotic process
• negative regulation of transcription by RNA polymerase II
• positive regulation of cell cycle

Molecular functions

• histone deacetylase binding

Cellular components

• nucleus

Protein domainsUniProt · Pfam · InterPro

• MAGE homology domain
• Melanoma associated antigen, N-terminal
• Melanoma-associated antigen
• MAGE homology domain, winged helix WH1 motif
• MAGE homology domain, winged helix WH2 motif
• MAGE homology domain
• Melanoma associated antigen family N terminal

KeywordsUniProt

• Tumor antigen

InteractionsUniProt · HPA

Protein binding partners of MAGEA4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ZBTB17
• AMOT
• PSMD10
• RAD18
• TIGD5
• TRIM69
• TSPYL1
• TSPYL4
• UQCRB