Seroatlas · Human Serome Atlas

RAD18

E3 ubiquitin-protein ligase RAD18

Also known as: RAD18_HUMAN, RNF73

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NS91
Gene: RAD18
Ensembl: ENSG00000070950
Chromosome: 3
Canonical length: 495 aa
Protein class: Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear speckles,Nuclear bodies
Quaternary structure: Homodimer

OverviewNCBI Gene

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

495 residues, UniProt reviewed canonical sequence.

>Q9NS91|RAD18
     1  MDSLAESRWP PGLAVMKTID DLLRCGICFE YFNIAMIIPQ CSHNYCSLCI RKFLSYKTQC
    61  PTCCVTVTEP DLKNNRILDE LVKSLNFARN HLLQFALESP AKSPASSSSK NLAVKVYTPV
   121  ASRQSLKQGS RLMDNFLIRE MSGSTSELLI KENKSKFSPQ KEASPAAKTK ETRSVEEIAP
   181  DPSEAKRPEP PSTSTLKQVT KVDCPVCGVN IPESHINKHL DSCLSREEKK ESLRSSVHKR
   241  KPLPKTVYNL LSDRDLKKKL KEHGLSIQGN KQQLIKRHQE FVHMYNAQCD ALHPKSAAEI
   301  VREIENIEKT RMRLEASKLN ESVMVFTKDQ TEKEIDEIHS KYRKKHKSEF QLLVDQARKG
   361  YKKIAGMSQK TVTITKEDES TEKLSSVCMG QEDNMTSVTN HFSQSKLDSP EELEPDREED
   421  SSSCIDIQEV LSSSESDSCN SSSSDIIRDL LEEEEAWEAS HKNDLQDTEI SPRQNRRTRA
   481  AESAEIEPRN KRNRN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RAD18 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.52
Highest tissue expression: 9.5 nTPM

Expression across tissuesHPA

Tissue

duodenum: 9.5 nTPM
small intestine: 6.1 nTPM
thymus: 5.4 nTPM
breast: 5 nTPM
bone marrow: 4.9 nTPM
rectum: 4.6 nTPM

Single-cell type

prostatic glandular cells: 75 nCPM
erythrocyte progenitors: 71 nCPM
megakaryocyte progenitors: 64 nCPM
early primary spermatocytes: 62 nCPM
brain inhibitory neurons: 54 nCPM
brain excitatory neurons: 53 nCPM

Immune cell

basophil: 6.4 nTPM
eosinophil: 5.2 nTPM
plasmacytoid DC: 3.6 nTPM
T-reg: 3 nTPM
MAIT T-cell: 2.9 nTPM
NK-cell: 2.8 nTPM

Brain region

white matter: 8.4 nTPM
cerebral cortex: 7.2 nTPM
medulla oblongata: 7 nTPM
basal ganglia: 6.9 nTPM
pons: 6.9 nTPM
cerebellum: 6.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.99
gnomAD pLI: 0
gnomAD missense Z: -0.14
DepMap mean gene effect: -0.09
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RAD18 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RAD18 as an antibody target. Whether an autoantibody or antibody against RAD18 could matter depends on whether native RAD18 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RAD18 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RAD18 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...