Seroatlas · Human Serome Atlas

TSPYL4

Testis-specific Y-encoded-like protein 4

Also known as: dJ486I3.2, KIAA0721, TSYL4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9UJ04
Gene: TSPYL4
Ensembl: ENSG00000187189
Chromosome: 6
Canonical length: 414 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Predicted to enable chromatin binding activity and histone binding activity. Predicted to be involved in nucleosome assembly. Predicted to be active in chromatin and nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

414 residues, UniProt reviewed canonical sequence.

>Q9UJ04|TSPYL4
     1  MSGLDGGNKL PLAQTGGLAA PDHASGDPDR DQCQGLREET EATQVMANTG GGSLETVAEG
    61  GASQDPVDCG PALRVPVAGS RGGAATKAGQ EDAPPSTKGL EAASAAEAAD SSQKNGCQLG
   121  EPRGPAGQKA LEACGAGGLG SQMIPGKKAK EVTTKKRAIS AAVEKEGEAG AAMEEKKVVQ
   181  KEKKVAGGVK EETRPRAPKI NNCMDSLEAI DQELSNVNAQ ADRAFLQLER KFGRMRRLHM
   241  QRRSFIIQNI PGFWVTAFRN HPQLSPMISG QDEDMLRYMI NLEVEELKHP RAGCKFKFIF
   301  QGNPYFRNEG LVKEYERRSS GRVVSLSTPI RWHRGQDPQA HIHRNREGNT IPSFFNWFSD
   361  HSLLEFDRIA EIIKGELWPN PLQYYLMGEG PRRGIRGPPR QPVESARSFR FQSG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TSPYL4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.56
Highest tissue expression: 89 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 89 nTPM
cerebral cortex: 75 nTPM
hypothalamus: 69 nTPM
basal ganglia: 66 nTPM
amygdala: 47 nTPM
hippocampal formation: 47 nTPM

Single-cell type

parietal cells: 58 nCPM
corticotrophs: 53 nCPM
adrenal medulla cells: 43 nCPM
other brain neurons: 40 nCPM
esophageal apical cells: 40 nCPM
fallopian tube ciliated cells: 38 nCPM

Immune cell

naive CD4 T-cell: 4.1 nTPM
total PBMC: 4 nTPM
memory CD4 T-cell: 3.9 nTPM
MAIT T-cell: 3.8 nTPM
NK-cell: 3.8 nTPM
T-reg: 3.7 nTPM

Brain region

hypothalamus: 167 nTPM
cerebral cortex: 166 nTPM
pons: 142 nTPM
basal ganglia: 141 nTPM
midbrain: 133 nTPM
cerebellum: 130 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.28
gnomAD pLI: 0
gnomAD missense Z: 0.74
DepMap mean gene effect: 0.02
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

nucleosome assembly

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

InteractionsUniProt · HPA

Protein binding partners of TSPYL4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

MAGEA4

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TSPYL4 as an antibody target. Whether an autoantibody or antibody against TSPYL4 could matter depends on whether native TSPYL4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TSPYL4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TSPYL4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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