Seroatlas · Human Serome Atlas

EMC4

ER membrane protein complex subunit 4

Also known as: EMC4_HUMAN, FLJ90746, MGC24415, PIG17, TMEM85

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5J8M3
Gene: EMC4
Ensembl: ENSG00000128463
Chromosome: 15
Canonical length: 183 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins, Transporters

OverviewNCBI Gene

Contributes to membrane insertase activity. Involved in protein insertion into ER membrane by stop-transfer membrane-anchor sequence and tail-anchored membrane protein insertion into ER membrane. Located in endoplasmic reticulum membrane. Part of EMC complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

183 residues, UniProt reviewed canonical sequence.

>Q5J8M3|EMC4
     1  MTAQGGLVAN RGRRFKWAIE LSGPGGGSRG RSDRGSGQGD SLYPVGYLDK QVPDTSVQET
    61  DRILVEKRCW DIALGPLKQI PMNLFIMYMA GNTISIFPTM MVCMMAWRPI QALMAISATF
   121  KMLESSSQKF LQGLVYLIGN LMGLALAVYK CQSMGLLPTH ASDWLAFIEP PERMEFSGGG
   181  LLL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EMC4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 3
Mean surface accessibility (rSASA): 0.5
Highest tissue expression: 201 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 201 nTPM
tongue: 128 nTPM
choroid plexus: 121 nTPM
epididymis: 97 nTPM
cerebral cortex: 96 nTPM
amygdala: 86 nTPM

Single-cell type

esophageal apical cells: 544 nCPM
esophageal suprabasal cells: 236 nCPM
late primary spermatocytes: 235 nCPM
extravillous trophoblasts: 219 nCPM
decidual stromal cells: 211 nCPM
epididymal principal cells: 203 nCPM

Immune cell

total PBMC: 278 nTPM
basophil: 257 nTPM
memory B-cell: 238 nTPM
T-reg: 217 nTPM
non-classical monocyte: 201 nTPM
NK-cell: 199 nTPM

Brain region

white matter: 76 nTPM
choroid plexus: 75 nTPM
cerebellum: 71 nTPM
cerebral cortex: 69 nTPM
hypothalamus: 69 nTPM
pons: 69 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.44
gnomAD pLI: 0.86
gnomAD missense Z: 1.28
DepMap mean gene effect: -0.41
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

ER membrane protein complex subunit 4
ER membrane protein complex subunit 4

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EMC4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EMC4 as an antibody target. Whether an autoantibody or antibody against EMC4 could matter depends on whether native EMC4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EMC4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EMC4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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