Seroatlas · Human Serome Atlas

ECSIT

Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial

Also known as: ECSIT_HUMAN, SITPEC

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9BQ95
Gene
ECSIT
Ensembl
ENSG00000130159
Chromosome
19
Canonical length
431 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol

OverviewNCBI Gene

Enables molecular adaptor activity. Involved in regulation of oxidoreductase activity; regulation of protein complex stability; and toll-like receptor 4 signaling pathway. Located in cytosol; mitochondrion; and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

431 residues, UniProt reviewed canonical sequence.

>Q9BQ95|ECSIT
     1  MSWVQATLLA RGLCRAWGGT CGAALTGTSI SQVPRRLPRG LHCSAAAHSS EQSLVPSPPE
    61  PRQRPTKALV PFEDLFGQAP GGERDKASFL QTVQKFAEHS VRKRGHIDFI YLALRKMREY
   121  GVERDLAVYN QLLNIFPKEV FRPRNIIQRI FVHYPRQQEC GIAVLEQMEN HGVMPNKETE
   181  FLLIQIFGRK SYPMLKLVRL KLWFPRFMNV NPFPVPRDLP QDPVELAMFG LRHMEPDLSA
   241  RVTIYQVPLP KDSTGAADPP QPHIVGIQSP DQQAALARHN PARPVFVEGP FSLWLRNKCV
   301  YYHILRADLL PPEEREVEET PEEWNLYYPM QLDLEYVRSG WDNYEFDINE VEEGPVFAMC
   361  MAGAHDQATM AKWIQGLQET NPTLAQIPVV FRLAGSTREL QTSSAGLEEP PLPEDHQEED
   421  DNLQRQQQGQ S

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ECSIT can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.44
Highest tissue expression
116 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 116 nTPM
  • tongue: 113 nTPM
  • heart muscle: 111 nTPM
  • pancreas: 48 nTPM
  • choroid plexus: 45 nTPM
  • liver: 44 nTPM

Single-cell type

  • esophageal basal cells: 100 nCPM
  • enteric stem cells: 96 nCPM
  • enteric transient amplifying cells: 96 nCPM
  • parietal cells: 90 nCPM
  • late spermatids: 85 nCPM
  • pancreatic acinar cells: 75 nCPM

Immune cell

  • myeloid DC: 41 nTPM
  • memory B-cell: 41 nTPM
  • intermediate monocyte: 39 nTPM
  • naive B-cell: 39 nTPM
  • non-classical monocyte: 37 nTPM
  • T-reg: 35 nTPM

Brain region

  • cerebellum: 41 nTPM
  • cerebral cortex: 36 nTPM
  • pons: 36 nTPM
  • choroid plexus: 36 nTPM
  • thalamus: 36 nTPM
  • medulla oblongata: 35 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.99
gnomAD pLI
0
gnomAD missense Z
0.18
DepMap mean gene effect
-0.19
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • ECSIT
  • ECSIT, C-terminal domain
  • ECSIT, N-terminal
  • Evolutionarily conserved signalling intermediate in Toll pathway
  • C-terminal domain of the ECSIT protein

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ECSIT in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ECSIT as an antibody target. Whether an autoantibody or antibody against ECSIT could matter depends on whether native ECSIT is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ECSIT is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ECSIT as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ECSIT. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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