Seroatlas · Human Serome Atlas

FARSA

Phenylalanine--tRNA ligase alpha subunit

Also known as: CML33, FARSL, FARSLA, SYFA_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9Y285
Gene
FARSA
Ensembl
ENSG00000179115
Chromosome
19
Canonical length
508 aa
Protein class
Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Potential drug targets, Predicted intracellular proteins
Subcellular location
Cytosol

OverviewNCBI Gene

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

508 residues, UniProt reviewed canonical sequence.

>Q9Y285|FARSA
     1  MADGQVAELL LRRLEASDGG LDSAELAAEL GMEHQAVVGA VKSLQALGEV IEAELRSTKH
    61  WELTAEGEEI AREGSHEARV FRSIPPEGLA QSELMRLPSG KVGFSKAMSN KWIRVDKSAA
   121  DGPRVFRVVD SMEDEVQRRL QLVRGGQAEK LGEKERSELR KRKLLAEVTL KTYWVSKGSA
   181  FSTSISKQET ELSPEMISSG SWRDRPFKPY NFLAHGVLPD SGHLHPLLKV RSQFRQIFLE
   241  MGFTEMPTDN FIESSFWNFD ALFQPQQHPA RDQHDTFFLR DPAEALQLPM DYVQRVKRTH
   301  SQGGYGSQGY KYNWKLDEAR KNLLRTHTTS ASARALYRLA QKKPFTPVKY FSIDRVFRNE
   361  TLDATHLAEF HQIEGVVADH GLTLGHLMGV LREFFTKLGI TQLRFKPAYN PYTEPSMEVF
   421  SYHQGLKKWV EVGNSGVFRP EMLLPMGLPE NVSVIAWGLS LERPTMIKYG INNIRELVGH
   481  KVNLQMVYDS PLCRLDAEPR PPPTQEAA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FARSA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.31
Highest tissue expression
58 nTPM

Expression across tissuesHPA

Tissue

  • cerebral cortex: 58 nTPM
  • skeletal muscle: 54 nTPM
  • hypothalamus: 41 nTPM
  • liver: 38 nTPM
  • esophagus: 38 nTPM
  • basal ganglia: 37 nTPM

Single-cell type

  • syncytiotrophoblasts: 123 nCPM
  • cytotrophoblasts: 107 nCPM
  • migrating cytotrophoblasts: 100 nCPM
  • extravillous trophoblasts: 94 nCPM
  • esophageal basal cells: 81 nCPM
  • esophageal suprabasal cells: 75 nCPM

Immune cell

  • total PBMC: 130 nTPM
  • myeloid DC: 121 nTPM
  • intermediate monocyte: 119 nTPM
  • NK-cell: 118 nTPM
  • classical monocyte: 111 nTPM
  • MAIT T-cell: 108 nTPM

Brain region

  • cerebral cortex: 67 nTPM
  • pons: 64 nTPM
  • hypothalamus: 62 nTPM
  • midbrain: 60 nTPM
  • medulla oblongata: 60 nTPM
  • basal ganglia: 59 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about FARSA.

Disease | AllUniProt

Conditions FARSA is implicated in, by any mechanism.

  • Rajab interstitial lung disease with brain calcifications 2 (RILDBC2) MIM:619013

Disease | GeneticClinVar

4 pathogenic / likely-pathogenic of 109 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Rajab interstitial lung disease with brain calcifications 2

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.53
gnomAD pLI
0.01
gnomAD missense Z
1.22
DepMap mean gene effect
-0.89
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of FARSA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads FARSA as an antibody target. Whether an autoantibody or antibody against FARSA could matter depends on whether native FARSA is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

FARSA is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label FARSA as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/FARSA. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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