MAP3K1

Protein identityUniProt · HPA

UniProt accession

Q13233

Gene

MAP3K1

Ensembl

ENSG00000095015

Chromosome

5

Canonical length

1512 aa

Protein class

Cancer-related genes, Disease related genes, Enzymes, FDA approved drug targets, Human disease related genes, Predicted membrane proteins

Subcellular location

Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012]

Canonical amino-acid sequenceUniProt

1512 residues, UniProt reviewed canonical sequence.

>Q13233|MAP3K1
     1  MAAAAGNRAS SSGFPGARAT SPEAGGGGGA LKASSAPAAA AGLLREAGSG GRERADWRRR
    61  QLRKVRSVEL DQLPEQPLFL AASPPASSTS PSPEPADAAG SGTGFQPVAV PPPHGAASRG
   121  GAHLTESVAA PDSGASSPAA AEPGEKRAPA AEPSPAAAPA GREMENKETL KGLHKMDDRP
   181  EERMIREKLK ATCMPAWKHE WLERRNRRGP VVVKPIPVKG DGSEMNHLAA ESPGEVQASA
   241  ASPASKGRRS PSPGNSPSGR TVKSESPGVR RKRVSPVPFQ SGRITPPRRA PSPDGFSPYS
   301  PEETNRRVNK VMRARLYLLQ QIGPNSFLIG GDSPDNKYRV FIGPQNCSCA RGTFCIHLLF
   361  VMLRVFQLEP SDPMLWRKTL KNFEVESLFQ KYHSRRSSRI KAPSRNTIQK FVSRMSNSHT
   421  LSSSSTSTSS SENSIKDEEE QMCPICLLGM LDEESLTVCE DGCRNKLHHH CMSIWAEECR
   481  RNREPLICPL CRSKWRSHDF YSHELSSPVD SPSSLRAAQQ QTVQQQPLAG SRRNQESNFN
   541  LTHYGTQQIP PAYKDLAEPW IQVFGMELVG CLFSRNWNVR EMALRRLSHD VSGALLLANG
   601  ESTGNSGGSS GSSPSGGATS GSSQTSISGD VVEACCSVLS MVCADPVYKV YVAALKTLRA
   661  MLVYTPCHSL AERIKLQRLL QPVVDTILVK CADANSRTSQ LSISTLLELC KGQAGELAVG
   721  REILKAGSIG IGGVDYVLNC ILGNQTESNN WQELLGRLCL IDRLLLEFPA EFYPHIVSTD
   781  VSQAEPVEIR YKKLLSLLTF ALQSIDNSHS MVGKLSRRIY LSSARMVTTV PHVFSKLLEM
   841  LSVSSSTHFT RMRRRLMAIA DEVEIAEAIQ LGVEDTLDGQ QDSFLQASVP NNYLETTENS
   901  SPECTVHLEK TGKGLCATKL SASSEDISER LASISVGPSS STTTTTTTTE QPKPMVQTKG
   961  RPHSQCLNSS PLSHHSQLMF PALSTPSSST PSVPAGTATD VSKHRLQGFI PCRIPSASPQ
  1021  TQRKFSLQFH RNCPENKDSD KLSPVFTQSR PLPSSNIHRP KPSRPTPGNT SKQGDPSKNS
  1081  MTLDLNSSSK CDDSFGCSSN SSNAVIPSDE TVFTPVEEKC RLDVNTELNS SIEDLLEASM
  1141  PSSDTTVTFK SEVAVLSPEK AENDDTYKDD VNHNQKCKEK MEAEEEEALA IAMAMSASQD
  1201  ALPIVPQLQV ENGEDIIIIQ QDTPETLPGH TKAKQPYRED TEWLKGQQIG LGAFSSCYQA
  1261  QDVGTGTLMA VKQVTYVRNT SSEQEEVVEA LREEIRMMSH LNHPNIIRML GATCEKSNYN
  1321  LFIEWMAGGS VAHLLSKYGA FKESVVINYT EQLLRGLSYL HENQIIHRDV KGANLLIDST
  1381  GQRLRIADFG AAARLASKGT GAGEFQGQLL GTIAFMAPEV LRGQQYGRSC DVWSVGCAII
  1441  EMACAKPPWN AEKHSNHLAL IFKIASATTA PSIPSHLSPG LRDVALRCLE LQPQDRPPSR
  1501  ELLKHPVFRT TW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAP3K1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.46

Highest tissue expression

25 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MAP3K1.

Disease | AllUniProt

Conditions MAP3K1 is implicated in, by any mechanism.

• 46,XY sex reversal 6 (SRXY6) MIM:613762

Disease | GeneticClinVar

18 pathogenic / likely-pathogenic of 470 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• 46,XY sex reversal 6
• Ovarian neoplasm
• MAP3K1-related disorder
• Inborn genetic diseases
• See cases

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.21

gnomAD pLI

1

gnomAD missense Z

1.67

DepMap mean gene effect

-0.16

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to mechanical stimulus
• Fc-epsilon receptor signaling pathway
• protein phosphorylation

Molecular functions

• ATP binding
• MAP kinase kinase kinase activity
• protein kinase activity
• protein kinase binding
• protein serine kinase activity
• protein serine/threonine kinase activity
• ubiquitin protein ligase activity
• zinc ion binding

Cellular components

• cytoplasm
• cytosol
• tight junction

Protein domainsUniProt · Pfam · InterPro

• Protein kinase domain
• Zinc finger, RING-type
• Zinc finger, SWIM-type
• Serine/threonine-protein kinase, active site
• Protein kinase-like domain superfamily
• Armadillo-like helical
• Zinc finger, RING/FYVE/PHD-type
• Armadillo-type fold
• Protein kinase, ATP binding site
• Mitogen-activated protein kinase kinase kinase
• Protein kinase domain
• SWIM zinc finger
• Centrosomal protein CEP104, TOG domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Kinase
• Magnesium
• Metal-binding
• Nucleotide-binding
• Phosphoprotein
• Serine/threonine-protein kinase
• Transferase
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of MAP3K1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• AXIN1
• GRIPAP1
• MAP4K1
• STK38
• ECSIT
• DCAF7
• MAP2K4
• TRAF2
• YWHAE