Seroatlas · Human Serome Atlas

DNAJC8

DnaJ homolog subfamily C member 8

Also known as: DNJC8_HUMAN, SPF31

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O75937
Gene: DNAJC8
Ensembl: ENSG00000126698
Chromosome: 1
Canonical length: 253 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Enables Hsp70 protein binding activity. Located in cytosol; intercellular bridge; and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

253 residues, UniProt reviewed canonical sequence.

>O75937|DNAJC8
     1  MAASGESGTS GGGGSTEEAF MTFYSEVKQI EKRDSVLTSK NQIERLTRPG SSYFNLNPFE
    61  VLQIDPEVTD EEIKKRFRQL SILVHPDKNQ DDADRAQKAF EAVDKAYKLL LDQEQKKRAL
   121  DVIQAGKEYV EHTVKERKKQ LKKEGKPTIV EEDDPELFKQ AVYKQTMKLF AELEIKRKER
   181  EAKEMHERKR QREEEIEAQE KAKREREWQK NFEESRDGRV DSWRNFQANT KGKKEKKNRT
   241  FLRPPKVKME QRE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNAJC8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 42 nTPM

Expression across tissuesHPA

Tissue

blood vessel: 42 nTPM
skeletal muscle: 42 nTPM
thymus: 40 nTPM
tonsil: 39 nTPM
heart muscle: 38 nTPM
lymph node: 38 nTPM

Single-cell type

megakaryocytes: 253 nCPM
syncytiotrophoblasts: 248 nCPM
hofbauer cells: 238 nCPM
migrating cytotrophoblasts: 211 nCPM
cytotrophoblasts: 208 nCPM
erythrocyte progenitors: 185 nCPM

Immune cell

total PBMC: 92 nTPM
T-reg: 67 nTPM
basophil: 64 nTPM
MAIT T-cell: 62 nTPM
plasmacytoid DC: 61 nTPM
gdT-cell: 61 nTPM

Brain region

cerebral cortex: 16 nTPM
pons: 14 nTPM
white matter: 14 nTPM
hypothalamus: 14 nTPM
medulla oblongata: 14 nTPM
cerebellum: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.59
gnomAD pLI: 0.05
gnomAD missense Z: 1.56
DepMap mean gene effect: -0.83
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

Hsp70 protein binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

DnaJ domain
Chaperone J-domain superfamily
DnaJ domain
DnaJ homolog subfamily C member 8

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DNAJC8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DNAJC8 as an antibody target. Whether an autoantibody or antibody against DNAJC8 could matter depends on whether native DNAJC8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DNAJC8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DNAJC8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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