RAP1A
Ras-related protein Rap-1A
Also known as: KREV-1, RAP1A_HUMAN, SMGP21
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P62834
- Gene
- RAP1A
- Ensembl
- ENSG00000116473
- Chromosome
- 1
- Canonical length
- 184 aa
- Protein class
- Enzymes, Predicted intracellular proteins, RAS pathway related proteins
OverviewNCBI Gene
This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Canonical amino-acid sequenceUniProt
184 residues, UniProt reviewed canonical sequence.
>P62834|RAP1A
1 MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG
61 TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL
121 EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS
181 CLLLLocalizationUniProt · AlphaFold · HPA
Whether an antibody against RAP1A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Cell surface
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.29
- Highest tissue expression
- 142 nTPM
Expression across tissuesHPA
Tissue
- blood vessel: 142 nTPM
- colon: 132 nTPM
- tonsil: 109 nTPM
- parathyroid gland: 107 nTPM
- lymph node: 102 nTPM
- esophagus: 94 nTPM
Single-cell type
- neutrophils: 1,211 nCPM
- neutrophil progenitors: 656 nCPM
- esophageal apical cells: 583 nCPM
- smooth muscle cells: 538 nCPM
- microglia: 535 nCPM
- kupffer cells: 467 nCPM
Immune cell
- neutrophil: 34 nTPM
- T-reg: 18 nTPM
- memory CD4 T-cell: 18 nTPM
- NK-cell: 16 nTPM
- intermediate monocyte: 15 nTPM
- memory CD8 T-cell: 15 nTPM
Brain region
- white matter: 78 nTPM
- medulla oblongata: 63 nTPM
- spinal cord: 57 nTPM
- cerebellum: 56 nTPM
- basal ganglia: 54 nTPM
- midbrain: 50 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.54
- gnomAD pLI
- 0.62
- gnomAD missense Z
- 2.36
- DepMap mean gene effect
- -0.1
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cellular response to cAMP
- cellular response to forskolin
- cellular response to nerve growth factor stimulus
- establishment of endothelial barrier
- liver regeneration
- negative regulation of collagen biosynthetic process
- negative regulation of synaptic vesicle exocytosis
- nerve growth factor signaling pathway
- nervous system development
- positive regulation of D-glucose import
- positive regulation of ERK1 and ERK2 cascade
- positive regulation of Fc receptor mediated stimulatory signaling pathway
- positive regulation of GTPase activity
- positive regulation of neuron projection development
- positive regulation of phagocytosis
- positive regulation of protein kinase activity
- positive regulation of vasculogenesis
- protein localization to plasma membrane
- Rap protein signal transduction
- regulation of cell junction assembly
- regulation of neurotransmitter receptor localization to postsynaptic specialization membrane
- response to antineoplastic agent
- response to carbohydrate
- synaptic vesicle exocytosis
Molecular functions
- G protein activity
- GDP binding
- GTP binding
- GTPase activity
- guanyl-nucleotide exchange factor activity
- protein-containing complex binding
- small GTPase binding
Cellular components
- anchoring junction
- cell junction
- cytoplasm
- cytosol
- early endosome
- endosome membrane
- extracellular exosome
- glutamatergic synapse
- guanyl-nucleotide exchange factor complex
- late endosome
- neuron projection
- perinuclear region of cytoplasm
- phagocytic vesicle
- plasma membrane
- presynapse
- specific granule membrane
- sperm midpiece
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of RAP1A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads RAP1A as an antibody target. Whether an autoantibody or antibody against RAP1A could matter depends on whether native RAP1A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
RAP1A is annotated at the cell surface, where native RAP1A is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.
Annotation status
The present source text does not explicitly label RAP1A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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