EBF1

Protein identityUniProt · HPA

UniProt accession

Q9UH73

Gene

EBF1

Ensembl

ENSG00000164330

Chromosome

5

Canonical length

591 aa

Protein class

Cancer-related genes, Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm,Vesicles

Quaternary structure

Homodimer

OverviewNCBI Gene

Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in positive regulation of transcription by RNA polymerase II. Predicted to act upstream of or within positive regulation of DNA-templated transcription. Predicted to be located in chromatin. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

591 residues, UniProt reviewed canonical sequence.

>Q9UH73|EBF1
     1  MFGIQESIQR SGSSMKEEPL GSGMNAVRTW MQGAGVLDAN TAAQSGVGLA RAHFEKQPPS
    61  NLRKSNFFHF VLALYDRQGQ PVEIERTAFV GFVEKEKEAN SEKTNNGIHY RLQLLYSNGI
   121  RTEQDFYVRL IDSMTKQAIV YEGQDKNPEM CRVLLTHEIM CSRCCDKKSC GNRNETPSDP
   181  VIIDRFFLKF FLKCNQNCLK NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH
   241  GRRARRLDPS EGTPSYLEHA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVIFGTMLV
   301  WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGTPG RFIYTALNEP TIDYGFQRLQ
   361  KVIPRHPGDP ERLPKEVILK RAADLVEALY GMPHNNQEII LKRAADIAEA LYSVPRNHNQ
   421  LPALANTSVH AGMMGVNSFS GQLAVNVSEA SQATNQGFTR NSSSVSPHGY VPSTTPQQTN
   481  YNSVTTSMNG YGSAAMSNLG GSPTFLNGSA ANSPYAIVPS SPTMASSTSL PSNCSSSSGI
   541  FSFSPANMVS AVKQKSAFAP VVRPQTSPPP TCTSTNGNSL QAISGMIVPP M

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EBF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.48

Highest tissue expression

35 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.26

gnomAD pLI

1

gnomAD missense Z

3.38

DepMap mean gene effect

-0.05

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• regulation of transcription by RNA polymerase II

Molecular functions

• DNA-binding transcription factor activity, RNA polymerase II-specific
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• zinc ion binding

Cellular components

• chromatin
• nucleus

Protein domainsUniProt · Pfam · InterPro

• IPT domain
• Transcription factor COE
• Immunoglobulin-like fold
• Immunoglobulin E-set
• Transcription factor COE, conserved site
• Transcription factor COE, DNA-binding domain
• Transcription factor COE, helix-loop-helix domain
• Transcription factor COE, IPT domain
• Transcription factor COE, DNA-binding domain superfamily
• IPT/TIG domain
• Transcription factor COE1 DNA-binding domain
• Transcription factor COE1 helix-loop-helix domain

KeywordsUniProt

• Acetylation
• Activator
• Developmental protein
• DNA-binding
• Isopeptide bond
• Metal-binding
• Nucleus
• Transcription
• Transcription regulation
• Ubl conjugation
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of EBF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ZNF423
• ZNF521
• CNOT3