Seroatlas · Human Serome Atlas

HELB

DNA helicase B

Also known as: HELB_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NG08
Gene: HELB
Ensembl: ENSG00000127311
Chromosome: 12
Canonical length: 1087 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear bodies,Mitochondria,Cytosol

OverviewNCBI Gene

This gene encodes a DNA-dependent ATPase which catalyzes the unwinding of DNA necessary for DNA replication, repair, recombination, and transcription. This gene is thought to function specifically during the S phase entry of the cell cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Canonical amino-acid sequenceUniProt

1087 residues, UniProt reviewed canonical sequence.

>Q8NG08|HELB
     1  MARSSPYLRQ LQGPLLPPRD LVEEDDDYLN DDVEEDEESV FIDAEELCSG GVKAGSLPGC
    61  LRVSICDENT QETCKVFGRF PITGAWWRVK VQVKPVVGSR SYQYQVQGFP SYFLQSDMSP
   121  PNQKHICALF LKECEVSSDD VNKFLTWVKE VSNYKNLNFE NLRETLRTFH KETGRKDQKQ
   181  PTQNGQEELF LDNEMSLPLE NTIPFRNVMT ALQFPKIMEF LPVLLPRHFK WIIGSGSKEM
   241  LKEIEEILGT HPWKLGFSKI TYREWKLLRC EASWIAFCQC ESLLQLMTDL EKNALIMYSR
   301  LKQICREDGH TYVEVNDLTL TLSNHMSFHA ASESLKFLKD IGVVTYEKSC VFPYDLYHAE
   361  RAIAFSICDL MKKPPWHLCV DVEKVLASIH TTKPENSSDD ALNESKPDEV RLENPVDVVD
   421  TQDNGDHIWT NGENEINAEI SEVQLDQDQV EVPLDRDQVA ALEMICSNPV TVISGKGGCG
   481  KTTIVSRLFK HIEQLEEREV KKACEDFEQD QNASEEWITF TEQSQLEADK AIEVLLTAPT
   541  GKAAGLLRQK TGLHAYTLCQ VNYSFYSWTQ TMMTTNKPWK FSSVRVLVVD EGSLVSVGIF
   601  KSVLNLLCEH SKLSKLIILG DIRQLPSIEP GNLLKDLFET LKSRNCAIEL KTNHRAESQL
   661  IVDNATRISR RQFPKFDAEL NISDNPTLPI SIQDKTFIFV RLPEEDASSQ SSKTNHHSCL
   721  YSAVKTLLQE NNLQNAKTSQ FIAFRRQDCD LINDCCCKHY TGHLTKDHQS RLVFGIGDKI
   781  CCTRNAYLSD LLPENISGSQ QNNDLDASSE DFSGTLPDFA KNKRDFESNV RLCNGEIFFI
   841  TNDVTDVTFG KRRSLTINNM AGLEVTVDFK KLMKYCRIKH AWARTIHTFQ GSEEQTVVYV
   901  VGKAGRQHWQ HVYTAVTRGR CRVYVIAEES QLRNAIMKNS FPRKTRLKHF LQSKLSSSGA
   961  PPADFPSPRK SSGDSGGPST PSASPLPVVT DHAMTNDVTW SEASSPDERT LTFAERWQLS
  1021  SPDGVDTDDD LPKSRASKRT CGVNDDESPS KIFMVGESPQ VSSRLQNLRL NNLIPRQLFK
  1081  PTDNQET

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HELB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.36
Highest tissue expression: 0.9 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 0.9 nTPM
lymph node: 0.5 nTPM
appendix: 0.4 nTPM
thymus: 0.4 nTPM
adrenal gland: 0.3 nTPM
spleen: 0.3 nTPM

Single-cell type

neutrophils: 291 nCPM
t-cells: 145 nCPM
nk-cells: 123 nCPM
salivary myoepithelial cells: 91 nCPM
monocyte progenitors: 89 nCPM
salivary acinar cells: 84 nCPM

Immune cell

eosinophil: 5.8 nTPM
T-reg: 5.6 nTPM
naive CD4 T-cell: 5.1 nTPM
memory CD4 T-cell: 5 nTPM
basophil: 4.7 nTPM
MAIT T-cell: 4.2 nTPM

Brain region

medulla oblongata: 1.3 nTPM
white matter: 1.1 nTPM
pons: 1 nTPM
cerebral cortex: 0.9 nTPM
thalamus: 0.9 nTPM
midbrain: 0.7 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.17
gnomAD pLI: 0
gnomAD missense Z: 0.39
DepMap mean gene effect: -0.13
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

DNA damage response
DNA repair
DNA replication
DNA replication, synthesis of primer
negative regulation of double-strand break repair via homologous recombination
regulation of DNA double-strand break processing

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

P-loop containing nucleoside triphosphate hydrolase
Coronaviruses polyprotein 1ab
DNA helicase B, winged helix domain
AAA domain
DNA helicase B winged helix domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of HELB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads HELB as an antibody target. Whether an autoantibody or antibody against HELB could matter depends on whether native HELB is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

HELB is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label HELB as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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