Seroatlas · Human Serome Atlas

ATG3

Ubiquitin-like-conjugating enzyme ATG3

Also known as: APG3L, ATG3_HUMAN, DKFZp564M1178, FLJ22125, MGC15201, PC3-96

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9NT62
Gene
ATG3
Ensembl
ENSG00000144848
Chromosome
3
Canonical length
314 aa
Protein class
Predicted intracellular proteins, Transporters
Subcellular location
Plasma membrane,Cytosol

OverviewNCBI Gene

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Canonical amino-acid sequenceUniProt

314 residues, UniProt reviewed canonical sequence.

>Q9NT62|ATG3
     1  MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE
    61  LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE DDGDGGWVDT YHNTGITGIT
   121  EAVKEITLEN KDNIRLQDCS ALCEEEEDED EGEAADMEEY EESGLLETDE ATLDTRKIVE
   181  ACKAKTDAGG EDAILQTRTY DLYITYDKYY QTPRLWLFGY DEQRQPLTVE HMYEDISQDH
   241  VKKTVTIENH PHLPPPPMCS VHPCRHAEVM KKIIETVAEG GGELGVHMYL LIFLKFVQAV
   301  IPTIEYDYTR HFTM

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ATG3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
89 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 89 nTPM
  • spinal cord: 80 nTPM
  • testis: 80 nTPM
  • midbrain: 57 nTPM
  • thymus: 48 nTPM
  • appendix: 46 nTPM

Single-cell type

  • early spermatids: 768 nCPM
  • late primary spermatocytes: 649 nCPM
  • neutrophils: 385 nCPM
  • oocytes: 327 nCPM
  • early primary spermatocytes: 193 nCPM
  • cdc: 174 nCPM

Immune cell

  • non-classical monocyte: 329 nTPM
  • intermediate monocyte: 258 nTPM
  • neutrophil: 174 nTPM
  • myeloid DC: 169 nTPM
  • classical monocyte: 168 nTPM
  • total PBMC: 138 nTPM

Brain region

  • white matter: 67 nTPM
  • cerebellum: 61 nTPM
  • medulla oblongata: 57 nTPM
  • basal ganglia: 57 nTPM
  • spinal cord: 54 nTPM
  • midbrain: 54 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.72
gnomAD pLI
0
gnomAD missense Z
1.76
DepMap mean gene effect
-0.12
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ATG3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ATG3 as an antibody target. Whether an autoantibody or antibody against ATG3 could matter depends on whether native ATG3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ATG3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ATG3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ATG3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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