Seroatlas · Human Serome Atlas

VPS28

Vacuolar protein sorting-associated protein 28 homolog

Also known as: VPS28_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9UK41
Gene
VPS28
Ensembl
ENSG00000160948
Chromosome
8
Canonical length
221 aa
Protein class
Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Canonical amino-acid sequenceUniProt

221 residues, UniProt reviewed canonical sequence.

>Q9UK41|VPS28
     1  MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC
    61  VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD
   121  DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV
   181  SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against VPS28 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.36
Highest tissue expression
182 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 182 nTPM
  • heart muscle: 166 nTPM
  • kidney: 163 nTPM
  • blood vessel: 154 nTPM
  • liver: 153 nTPM
  • ovary: 148 nTPM

Single-cell type

  • late primary spermatocytes: 297 nCPM
  • platelets: 275 nCPM
  • enterocytes: 254 nCPM
  • megakaryocytes: 173 nCPM
  • late spermatids: 171 nCPM
  • gastric chief cells: 163 nCPM

Immune cell

  • neutrophil: 0.8 nTPM
  • basophil: 0.7 nTPM
  • eosinophil: 0.3 nTPM
  • NK-cell: 0.3 nTPM
  • classical monocyte: 0.1 nTPM
  • gdT-cell: 0.1 nTPM

Brain region

  • pons: 1.2 nTPM
  • hypothalamus: 0.5 nTPM
  • medulla oblongata: 0.5 nTPM
  • midbrain: 0.5 nTPM
  • amygdala: 0.4 nTPM
  • cerebral cortex: 0.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.75
gnomAD pLI
0.07
gnomAD missense Z
0.87
DepMap mean gene effect
-1.67
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 12% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • ESCRT assembly domain
  • Vacuolar protein sorting-associated Vps28
  • Vacuolar protein sorting-associated, VPS28, N-terminal
  • Vacuolar protein sorting-associated, VPS28, C-terminal
  • VPS28, C-terminal domain superfamily
  • VPS28, N-terminal domain superfamily
  • VPS28 protein

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of VPS28 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads VPS28 as an antibody target. Whether an autoantibody or antibody against VPS28 could matter depends on whether native VPS28 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

VPS28 is annotated at the cell surface, where native VPS28 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label VPS28 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/VPS28. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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