Seroatlas · Human Serome Atlas

VPS25

Vacuolar protein-sorting-associated protein 25

Also known as: DERP9, EAP20, MGC10540, VPS25_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9BRG1
Gene
VPS25
Ensembl
ENSG00000131475
Chromosome
17
Canonical length
176 aa
Protein class
Predicted intracellular proteins
Subcellular location
Vesicles

OverviewNCBI Gene

This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]

Canonical amino-acid sequenceUniProt

176 residues, UniProt reviewed canonical sequence.

>Q9BRG1|VPS25
     1  MAMSFEWPWQ YRFPPFFTLQ PNVDTRQKQL AAWCSLVLSF CRLHKQSSMT VMEAQESPLF
    61  NNVKLQRKLP VESIQIVLEE LRKKGNLEWL DKSKSSFLIM WRRPEEWGKL IYQWVSRSGQ
   121  NNSVFTLYEL TNGEDTEDEE FHGLDEATLL RALQALQQEH KAEIITVSDG RGVKFF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against VPS25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.3
Highest tissue expression
90 nTPM

Expression across tissuesHPA

Tissue

  • esophagus: 90 nTPM
  • kidney: 69 nTPM
  • parathyroid gland: 68 nTPM
  • liver: 67 nTPM
  • rectum: 64 nTPM
  • epididymis: 62 nTPM

Single-cell type

  • esophageal suprabasal cells: 289 nCPM
  • esophageal apical cells: 277 nCPM
  • extravillous trophoblasts: 240 nCPM
  • migrating cytotrophoblasts: 163 nCPM
  • cytotrophoblasts: 155 nCPM
  • esophageal basal cells: 149 nCPM

Immune cell

  • eosinophil: 107 nTPM
  • intermediate monocyte: 89 nTPM
  • basophil: 86 nTPM
  • non-classical monocyte: 83 nTPM
  • myeloid DC: 80 nTPM
  • NK-cell: 79 nTPM

Brain region

  • choroid plexus: 29 nTPM
  • cerebellum: 25 nTPM
  • thalamus: 24 nTPM
  • white matter: 24 nTPM
  • medulla oblongata: 23 nTPM
  • spinal cord: 23 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about VPS25.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 23 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • VPS25-related neurodevelopmental delay

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.73
gnomAD pLI
0.08
gnomAD missense Z
1.48
DepMap mean gene effect
-2.2
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of VPS25 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads VPS25 as an antibody target. Whether an autoantibody or antibody against VPS25 could matter depends on whether native VPS25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

VPS25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label VPS25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/VPS25. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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