Seroatlas · Human Serome Atlas

U2AF1

Splicing factor U2AF 35 kDa subunit

Also known as: RN, RNU2AF1, U2AF1_HUMAN, U2AF35, U2AFBP

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q01081
Gene
U2AF1
Ensembl
ENSG00000160201
Chromosome
21
Canonical length
240 aa
Protein class
Cancer-related genes, Disease related genes, Human disease related genes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

240 residues, UniProt reviewed canonical sequence.

>Q01081|U2AF1
     1  MAEYLASIFG TEKDKVNCSF YFKIGACRHG DRCSRLHNKP TFSQTIALLN IYRNPQNSSQ
    61  SADGLRCAVS DVEMQEHYDE FFEEVFTEME EKYGEVEEMN VCDNLGDHLV GNVYVKFRRE
   121  EDAEKAVIDL NNRWFNGQPI HAELSPVTDF REACCRQYEM GECTRGGFCN FMHLKPISRE
   181  LRRELYGRRR KKHRSRSRSR ERRSRSRDRG RGGGGGGGGG GGGRERDRRR SRDRERSGRF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against U2AF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.41
Highest tissue expression
133 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 133 nTPM
  • thymus: 131 nTPM
  • tonsil: 118 nTPM
  • lymph node: 89 nTPM
  • ovary: 85 nTPM
  • urinary bladder: 82 nTPM

Single-cell type

  • neutrophils: 33 nCPM
  • enterocytes: 28 nCPM
  • pdcs: 23 nCPM
  • epididymal principal cells: 23 nCPM
  • breast lactating cells: 19 nCPM
  • mast cells: 19 nCPM

Immune cell

  • neutrophil: 518 nTPM
  • plasmacytoid DC: 514 nTPM
  • total PBMC: 393 nTPM
  • basophil: 374 nTPM
  • T-reg: 372 nTPM
  • naive B-cell: 353 nTPM

Brain region

  • white matter: 41 nTPM
  • cerebral cortex: 39 nTPM
  • hypothalamus: 38 nTPM
  • cerebellum: 37 nTPM
  • medulla oblongata: 36 nTPM
  • thalamus: 36 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about U2AF1.

Disease | AllUniProt

Conditions U2AF1 is implicated in, by any mechanism.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.22
gnomAD pLI
0.99
gnomAD missense Z
3.85
DepMap mean gene effect
-1.74
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of U2AF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads U2AF1 as an antibody target. Whether an autoantibody or antibody against U2AF1 could matter depends on whether native U2AF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

U2AF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label U2AF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/U2AF1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...