Seroatlas · Human Serome Atlas

TUBB3

Tubulin beta-3 chain

Also known as: beta-4, CFEOM3, CFEOM3A, FEOM3, TBB3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q13509
Gene
TUBB3
Ensembl
ENSG00000258947
Chromosome
16
Canonical length
450 aa
Protein class
Disease related genes, FDA approved drug targets, Human disease related genes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Microtubules,Cytokinetic bridge,Mitotic spindle,Primary cilium,Basal body,Flagellar centriole,Principal piece,End piece

OverviewNCBI Gene

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Canonical amino-acid sequenceUniProt

450 residues, UniProt reviewed canonical sequence.

>Q13509|TUBB3
     1  MREIVHIQAG QCGNQIGAKF WEVISDEHGI DPSGNYVGDS DLQLERISVY YNEASSHKYV
    61  PRAILVDLEP GTMDSVRSGA FGHLFRPDNF IFGQSGAGNN WAKGHYTEGA ELVDSVLDVV
   121  RKECENCDCL QGFQLTHSLG GGTGSGMGTL LISKVREEYP DRIMNTFSVV PSPKVSDTVV
   181  EPYNATLSIH QLVENTDETY CIDNEALYDI CFRTLKLATP TYGDLNHLVS ATMSGVTTSL
   241  RFPGQLNADL RKLAVNMVPF PRLHFFMPGF APLTARGSQQ YRALTVPELT QQMFDAKNMM
   301  AACDPRHGRY LTVATVFRGR MSMKEVDEQM LAIQSKNSSY FVEWIPNNVK VAVCDIPPRG
   361  LKMSSTFIGN STAIQELFKR ISEQFTAMFR RKAFLHWYTG EGMDEMEFTE AESNMNDLVS
   421  EYQQYQDATA EEEGEMYEDD EEESEAQGPK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TUBB3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.22
Highest tissue expression
344 nTPM

Expression across tissuesHPA

Tissue

  • hypothalamus: 344 nTPM
  • midbrain: 215 nTPM
  • cerebral cortex: 210 nTPM
  • cerebellum: 128 nTPM
  • hippocampal formation: 126 nTPM
  • amygdala: 124 nTPM

Single-cell type

  • other brain neurons: 291 nCPM
  • brain inhibitory neurons: 89 nCPM
  • brain excitatory neurons: 88 nCPM
  • astrocytes: 19 nCPM
  • oligodendrocyte progenitor cells: 19 nCPM
  • ependymal cells: 12 nCPM

Immune cell

  • naive B-cell: 2.5 nTPM
  • memory B-cell: 0.3 nTPM
  • plasmacytoid DC: 0.2 nTPM
  • non-classical monocyte: 0.1 nTPM
  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM

Brain region

  • hypothalamus: 691 nTPM
  • pons: 594 nTPM
  • medulla oblongata: 462 nTPM
  • thalamus: 408 nTPM
  • midbrain: 303 nTPM
  • cerebral cortex: 282 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about TUBB3.

Disease | AllUniProt

Conditions TUBB3 is implicated in, by any mechanism.

  • Fibrosis of extraocular muscles, congenital, 3A (CFEOM3A) MIM:600638
  • Cortical dysplasia, complex, with other brain malformations 1 (CDCBM1) MIM:614039

Disease | GeneticClinVar

54 pathogenic / likely-pathogenic of 384 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Complex cortical dysplasia with other brain malformations 1
  • Fibrosis of extraocular muscles, congenital, 3A, with or without extraocular involvement
  • TUBB3-related disorder
  • Inborn genetic diseases
  • TUBB3-related tubulinopathy

Disease | ImmuneIEDB

Conditions an epitope on TUBB3 was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.32
gnomAD pLI
0.97
gnomAD missense Z
4.58
DepMap mean gene effect
-0.06
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TUBB3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TUBB3 as an antibody target. Whether an autoantibody or antibody against TUBB3 could matter depends on whether native TUBB3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TUBB3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TUBB3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TUBB3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...