Seroatlas · Human Serome Atlas

CFAP61

Cilia- and flagella-associated protein 61

Also known as: C20orf26, CaM-IP3, CFA61_HUMAN, dJ1002M8.3, DKFZP434K156

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NHU2
Gene: CFAP61
Ensembl: ENSG00000089101
Chromosome: 20
Canonical length: 1237 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Nucleoplasm,Cytosol,Principal piece

OverviewNCBI Gene

Involved in sperm flagellum assembly. Predicted to be located in motile cilium and radial spoke stalk. Predicted to be active in sperm flagellum. Implicated in spermatogenic failure 84. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1237 residues, UniProt reviewed canonical sequence.

>Q8NHU2|CFAP61
     1  MSVLTSPRGK VEVVHCRRTE SQDVYCIKSL IRKFTCKLFG KLNIIYLLEK ANLAVTLCND
    61  KEEIMAQATF LDYPNWNVAK QDDWVSVFRE LDSDIPCTPL NTLFMHLFVA VDEYSVGCCK
   121  EILRTVYKAV PELHFIFLIV PSYMSLGSTL ITVFDQVGNI PCLTYEEDFA VHICHRHSHY
   181  PQLHVRKARV EDHDDLMPIF MRYDTILKET YGEYFLAELI EAQDEENHAV VCEVEGTAVG
   241  FMSVCSRVNM QLLHECFDLG PFHGLCFPHP DDVLESPQDL SVRRSQDAEL RSSSQGSQKI
   301  VEELQEPVSP DTMENIQGNI AREAASEEAL TAVQSGNVSE PEDIEKLSDI STGYAQYHHV
   361  SSRSLASLVL PEEPVHFRPI YRGASAAFCI QLFCIDEKYE ARSLDFMNFV FSLFSDKNFC
   421  VISLPHLTPE FFLIQNFVKM VPFNTCTLEQ DLYVFHRAGL LKSINIRFAT LLDTPGVENL
   481  VSTLMLNKSI LEDLDRYNKA RKDPDGTLLQ AFVAEVAEQI VGIAVIRNEM DIEYIRSHYN
   541  IEDFIYFSHH QREEHGHMHH FALNPIFRHY TKFFLKEILR LGFKSCLYYR VYPKSREGKF
   601  QNPYAHSLTS ALHYLVPVRP RRQIVYPLEK LGINAPSKAV SKDPMSYALN HTNRKLTLEP
   661  KITVNAKIIV VGASSVGISF LETLVFCSHM KFNNLTLIST HGLPGKKLLD TEQRKFLASD
   721  HCFNDKDYAL MSLCSWVNVV VGRMTGIDRA AKHVVLSTDE IVPYDHLILC TGQQYQVPCP
   781  TEADISQHLT NREVPNSSQR RYTGKVPCNH FTLNEEEDCF KALIWIRNNS ITTEGNIIVY
   841  GNTIDTYTTV ETLLNLGVSG SRIHLVQPPP ASTITCINNY SVESAVADAL GAAGVTMYRD
   901  AILAQWNDGL HPDPIYSASF TTPTKPFRLQ CSMFFSFCEK NVDYETFKAL NDACLVYDSR
   961  LVIDTNFHTN DIAIRAAGSL TKFSNRYYSN EWTHSNFSSK EIGFQLAAAM LHLFDPTLEP
  1021  VTEPPANLDR LIPMYKGAKI QGGILPGSYH YLHIAKPAIP TPLEVQMAQP NYGLELVTGS
  1081  AKNGTYFRIH INKYKMVETI TCLSREPFPA SNYIRLFGQH EQLLNNLCAR YDENLITDLY
  1141  SYFTEPWCLA LFHDRFIDLR KELRQILASK EEEDLPSIEQ LAHQIEDEEI NPTEKPRQYL
  1201  KRVFEESIYK TLVERSTLDY LHYNRYHLPM YAWPGIV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CFAP61 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.28
Highest tissue expression: 26 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 26 nTPM
testis: 15 nTPM
heart muscle: 12 nTPM
fallopian tube: 11 nTPM
tongue: 9.5 nTPM
choroid plexus: 6.5 nTPM

Single-cell type

ependymal cells: 600 nCPM
thymic myoid cells: 477 nCPM
respiratory ciliated cells: 477 nCPM
choroid plexus epithelial cells: 315 nCPM
cardiomyocytes: 287 nCPM
somatotrophs: 242 nCPM

Immune cell

gdT-cell: 0.5 nTPM
plasmacytoid DC: 0.4 nTPM
NK-cell: 0.2 nTPM
memory CD8 T-cell: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM

Brain region

choroid plexus: 37 nTPM
midbrain: 18 nTPM
medulla oblongata: 14 nTPM
spinal cord: 12 nTPM
hypothalamus: 10 nTPM
white matter: 7.5 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CFAP61.

Disease | AllUniProt

Conditions CFAP61 is implicated in, by any mechanism.

Spermatogenic failure 84 (SPGF84) MIM:620409

Disease | GeneticClinVar

9 pathogenic / likely-pathogenic of 240 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Spermatogenic failure 84
Situs inversus

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.73
gnomAD pLI: 0
DepMap mean gene effect: 0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

FAD/NAD(P)-binding domain superfamily
Cilia- and flagella-associated protein 61, N-terminal domain
Cilia- and flagella-associated protein 61
CFAP61, dimerisation domain
Cilia- and flagella-associated protein 61, N-terminal domain
CFAP61 dimerisation domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CFAP61 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CFAP61 as an antibody target. Whether an autoantibody or antibody against CFAP61 could matter depends on whether native CFAP61 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CFAP61 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CFAP61 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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