Seroatlas · Human Serome Atlas

SPSB4

SPRY domain-containing SOCS box protein 4

Also known as: SPSB4_HUMAN, SSB-4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96A44
Gene: SPSB4
Ensembl: ENSG00000175093
Chromosome: 3
Canonical length: 273 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Golgi apparatus

OverviewNCBI Gene

Enables ubiquitin-like ligase-substrate adaptor activity. Involved in several processes, including positive regulation of protein polyubiquitination; protein ubiquitination; and ubiquitin-dependent protein catabolic process. Located in cytosol. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

273 residues, UniProt reviewed canonical sequence.

>Q96A44|SPSB4
     1  MGQKLSGSLK SVEVREPALR PAKRELRGAE PGRPARLDQL LDMPAAGLAV QLRHAWNPED
    61  RSLNVFVKDD DRLTFHRHPV AQSTDGIRGK VGHARGLHAW QINWPARQRG THAVVGVATA
   121  RAPLHSVGYT ALVGSDAESW GWDLGRSRLY HDGKNQPGVA YPAFLGPDEA FALPDSLLVV
   181  LDMDEGTLSF IVDGQYLGVA FRGLKGKKLY PVVSAVWGHC EVTMRYINGL DPEPLPLMDL
   241  CRRSIRSALG RQRLQDISSL PLPQSLKNYL QYQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPSB4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.29
Highest tissue expression: 12 nTPM

Expression across tissuesHPA

Tissue

pancreas: 12 nTPM
testis: 6.9 nTPM
choroid plexus: 4.7 nTPM
heart muscle: 3.8 nTPM
pituitary gland: 2.6 nTPM
spleen: 2.6 nTPM

Single-cell type

choroid plexus epithelial cells: 83 nCPM
oligodendrocyte progenitor cells: 76 nCPM
lactotrophs: 75 nCPM
kupffer cells: 59 nCPM
megakaryocytes: 56 nCPM
gonadotrophs: 51 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

choroid plexus: 7.8 nTPM
medulla oblongata: 7.2 nTPM
hypothalamus: 6 nTPM
midbrain: 5.8 nTPM
pons: 4.7 nTPM
spinal cord: 4.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.41
gnomAD pLI: 0
gnomAD missense Z: 0.87
DepMap mean gene effect: 0.11
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

ubiquitin-like ligase-substrate adaptor activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SPSB4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPSB4 as an antibody target. Whether an autoantibody or antibody against SPSB4 could matter depends on whether native SPSB4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPSB4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SPSB4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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