Seroatlas · Human Serome Atlas

SART1

U4/U6.U5 tri-snRNP-associated protein 1

Also known as: Ara1, HAF, SNRNP110, Snu66, SNUT1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O43290
Gene: SART1
Ensembl: ENSG00000175467
Chromosome: 11
Canonical length: 800 aa
Protein class: Cancer-related genes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Nuclear speckles,Golgi apparatus

OverviewNCBI Gene

This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

800 residues, UniProt reviewed canonical sequence.

>O43290|SART1
     1  MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG ERRKRSRERG
    61  GERGSGRRGA EAEARSSTHG RERSQAEPSE RRVKREKRDD GYEAAASSKT SSGDASSLSI
   121  EETNKLRAKL GLKPLEVNAI KKEAGTKEEP VTADVINPMA LRQREELREK LAAAKEKRLL
   181  NQKLGKIKTL GEDDPWLDDT AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE
   241  FGQRRQDLYS ARDLQGLTVE HAIDSFREGE TMILTLKDKG VLQEEEDVLV NVNLVDKERA
   301  EKNVELRKKK PDYLPYAEDE SVDDLAQQKP RSILSKYDEE LEGERPHSFR LEQGGTADGL
   361  RERELEEIRA KLRLQAQSLS TVGPRLASEY LTPEEMVTFK KTKRRVKKIR KKEKEVVVRA
   421  DDLLPLGDQT QDGDFGSRLR GRGRRRVSEV EEEKEPVPQP LPSDDTRVEN MDISDEEEGG
   481  APPPGSPQVL EEDEAELELQ KQLEKGRRLR QLQQLQQLRD SGEKVVEIVK KLESRQRGWE
   541  EDEDPERKGA IVFNATSEFC RTLGEIPTYG LAGNREEQEE LMDFERDEER SANGGSESDG
   601  EENIGWSTVN LDEEKQQQDF SASSTTILDE EPIVNRGLAA ALLLCQNKGL LETTVQKVAR
   661  VKAPNKSLPS AVYCIEDKMA IDDKYSRREE YRGFTQDFKE KDGYKPDVKI EYVDETGRKL
   721  TPKEAFRQLS HRFHGKGSGK MKTERRMKKL DEEALLKKMS SSDTPLGTVA LLQEKQKAQK
   781  TPYIVLSGSG KSMNANTITK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SART1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.6
Highest tissue expression: 39 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 39 nTPM
skeletal muscle: 33 nTPM
spleen: 28 nTPM
skin: 28 nTPM
ovary: 25 nTPM
endometrium: 25 nTPM

Single-cell type

neutrophils: 71 nCPM
syncytiotrophoblasts: 62 nCPM
erythrocyte progenitors: 53 nCPM
retinal pigment epithelial cells: 51 nCPM
early primary spermatocytes: 47 nCPM
cytotrophoblasts: 47 nCPM

Immune cell

eosinophil: 8.1 nTPM
T-reg: 5.4 nTPM
memory CD4 T-cell: 4.8 nTPM
memory CD8 T-cell: 4.4 nTPM
gdT-cell: 4.3 nTPM
neutrophil: 4 nTPM

Brain region

white matter: 23 nTPM
basal ganglia: 22 nTPM
medulla oblongata: 22 nTPM
cerebral cortex: 21 nTPM
midbrain: 21 nTPM
hypothalamus: 21 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about SART1.

Disease | ImmuneIEDB

Conditions an epitope on SART1 was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.34
gnomAD pLI: 0.88
gnomAD missense Z: 2.3
DepMap mean gene effect: -1.11
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

RNA binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

SNU66/SART1 family
HIND motif
SART-1 family
HIND motif

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SART1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SART1 as an antibody target. Whether an autoantibody or antibody against SART1 could matter depends on whether native SART1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SART1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SART1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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