Seroatlas · Human Serome Atlas

RFC5

Replication factor C subunit 5

Also known as: RFC36, RFC5_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P40937
Gene
RFC5
Ensembl
ENSG00000111445
Chromosome
12
Canonical length
340 aa
Protein class
Metabolic proteins, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

This gene encodes the smallest subunit of the replication factor C complex, which consists of five distinct subunits (140, 40, 38, 37, and 36 kDa) and is required for DNA replication. This subunit interacts with the C-terminal region of proliferating cell nuclear antigen and is required to open and load proliferating cell nuclear antigen onto DNA during S phase. It is a member of the AAA+ (ATPases associated with various cellular activities) ATPase family and forms a core complex with the 38 and 40 kDa subunits that possesses DNA-dependent ATPase activity. A related pseudogene has been identified on chromosome 9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]

Canonical amino-acid sequenceUniProt

340 residues, UniProt reviewed canonical sequence.

>P40937|RFC5
     1  METSALKQQE QPAATKIRNL PWVEKYRPQT LNDLISHQDI LSTIQKFINE DRLPHLLLYG
    61  PPGTGKTSTI LACAKQLYKD KEFGSMVLEL NASDDRGIDI IRGPILSFAS TRTIFKKGFK
   121  LVILDEADAM TQDAQNALRR VIEKFTENTR FCLICNYLSK IIPALQSRCT RFRFGPLTPE
   181  LMVPRLEHVV EEEKVDISED GMKALVTLSS GDMRRALNIL QSTNMAFGKV TEETVYTCTG
   241  HPLKSDIANI LDWMLNQDFT TAYRNITELK TLKGLALHDI LTEIHLFVHR VDFPSSVRIH
   301  LLTKMADIEY RLSVGTNEKI QLSSLIAAFQ VTRDLIVAEA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RFC5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.3
Highest tissue expression
31 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 31 nTPM
  • thymus: 22 nTPM
  • lymph node: 15 nTPM
  • tonsil: 15 nTPM
  • ovary: 13 nTPM
  • liver: 12 nTPM

Single-cell type

  • erythrocyte progenitors: 60 nCPM
  • esophageal basal cells: 42 nCPM
  • extravillous trophoblasts: 39 nCPM
  • migrating cytotrophoblasts: 37 nCPM
  • cytotrophoblasts: 33 nCPM
  • epicardial cells: 30 nCPM

Immune cell

  • basophil: 17 nTPM
  • T-reg: 11 nTPM
  • memory B-cell: 11 nTPM
  • memory CD4 T-cell: 10 nTPM
  • naive B-cell: 9.1 nTPM
  • gdT-cell: 8.6 nTPM

Brain region

  • hippocampal formation: 15 nTPM
  • cerebellum: 13 nTPM
  • spinal cord: 13 nTPM
  • cerebral cortex: 12 nTPM
  • white matter: 12 nTPM
  • medulla oblongata: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.75
gnomAD pLI
0
gnomAD missense Z
1.22
DepMap mean gene effect
-1.46
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RFC5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RFC5 as an antibody target. Whether an autoantibody or antibody against RFC5 could matter depends on whether native RFC5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RFC5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RFC5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RFC5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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