Seroatlas · Human Serome Atlas

RFC3

Replication factor C subunit 3

Also known as: MGC5276, RFC3_HUMAN, RFC38

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P40938
Gene: RFC3
Ensembl: ENSG00000133119
Chromosome: 13
Canonical length: 356 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Vesicles

OverviewNCBI Gene

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

356 residues, UniProt reviewed canonical sequence.

>P40938|RFC3
     1  MSLWVDKYRP CSLGRLDYHK EQAAQLRNLV QCGDFPHLLV YGPSGAGKKT RIMCILRELY
    61  GVGVEKLRIE HQTITTPSKK KIEISTIASN YHLEVNPSDA GNSDRVVIQE MLKTVAQSQQ
   121  LETNSQRDFK VVLLTEVDKL TKDAQHALRR TMEKYMSTCR LILCCNSTSK VIPPIRSRCL
   181  AVRVPAPSIE DICHVLSTVC KKEGLNLPSQ LAHRLAEKSC RNLRKALLMC EACRVQQYPF
   241  TADQEIPETD WEVYLRETAN AIVSQQTPQR LLEVRGRLYE LLTHCIPPEI IMKGLLSELL
   301  HNCDGQLKGE VAQMAAYYEH RLQLGSKAIY HLEAFVAKFM ALYKKFMEDG LEGMMF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RFC3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.28
Highest tissue expression: 23 nTPM

Expression across tissuesHPA

Tissue

lymph node: 23 nTPM
thymus: 23 nTPM
tonsil: 22 nTPM
bone marrow: 18 nTPM
adrenal gland: 13 nTPM
appendix: 12 nTPM

Single-cell type

erythrocyte progenitors: 182 nCPM
adrenal cortex cells: 159 nCPM
undifferentiated spermatogonia: 152 nCPM
early primary spermatocytes: 133 nCPM
myonuclei: 103 nCPM
monocyte progenitors: 103 nCPM

Immune cell

eosinophil: 7.2 nTPM
T-reg: 6.3 nTPM
intermediate monocyte: 6.1 nTPM
myeloid DC: 6.1 nTPM
memory B-cell: 5.8 nTPM
naive B-cell: 5.5 nTPM

Brain region

cerebellum: 18 nTPM
spinal cord: 7.7 nTPM
pons: 5.8 nTPM
medulla oblongata: 5.7 nTPM
cerebral cortex: 5.6 nTPM
amygdala: 5.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.94
gnomAD pLI: 0
gnomAD missense Z: -0.13
DepMap mean gene effect: -1.42
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

AAA+ ATPase domain
DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal
P-loop containing nucleoside triphosphate hydrolase
DNA Replication and Repair Clamp Loader Complex
RCF1/5-like, AAA+ ATPase lid domain
DNA polymerase III, delta subunit
Replication factor C subunit 3, C-terminal domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RFC3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RFC3 as an antibody target. Whether an autoantibody or antibody against RFC3 could matter depends on whether native RFC3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RFC3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RFC3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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