Seroatlas · Human Serome Atlas

RFC4

Replication factor C subunit 4

Also known as: A1, RFC37, RFC4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P35249
Gene
RFC4
Ensembl
ENSG00000163918
Chromosome
3
Canonical length
363 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

363 residues, UniProt reviewed canonical sequence.

>P35249|RFC4
     1  MQAFLKGTSI STKPPLTKDR GVAASAGSSG ENKKAKPVPW VEKYRPKCVD EVAFQEEVVA
    61  VLKKSLEGAD LPNLLFYGPP GTGKTSTILA AARELFGPEL FRLRVLELNA SDERGIQVVR
   121  EKVKNFAQLT VSGSRSDGKP CPPFKIVILD EADSMTSAAQ AALRRTMEKE SKTTRFCLIC
   181  NYVSRIIEPL TSRCSKFRFK PLSDKIQQQR LLDIAKKENV KISDEGIAYL VKVSEGDLRK
   241  AITFLQSATR LTGGKEITEK VITDIAGVIP AEKIDGVFAA CQSGSFDKLE AVVKDLIDEG
   301  HAATQLVNQL HDVVVENNLS DKQKSIITEK LAEVDKCLAD GADEHLQLIS LCATVMQQLS
   361  QNC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RFC4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.33
Highest tissue expression
39 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 39 nTPM
  • thymus: 33 nTPM
  • tonsil: 32 nTPM
  • lymph node: 29 nTPM
  • testis: 21 nTPM
  • rectum: 19 nTPM

Single-cell type

  • early primary spermatocytes: 99 nCPM
  • erythrocyte progenitors: 94 nCPM
  • extravillous trophoblasts: 65 nCPM
  • oocytes: 65 nCPM
  • late primary spermatocytes: 62 nCPM
  • migrating cytotrophoblasts: 57 nCPM

Immune cell

  • T-reg: 22 nTPM
  • naive B-cell: 15 nTPM
  • non-classical monocyte: 15 nTPM
  • memory B-cell: 13 nTPM
  • memory CD8 T-cell: 12 nTPM
  • gdT-cell: 12 nTPM

Brain region

  • cerebellum: 8.3 nTPM
  • cerebral cortex: 7.2 nTPM
  • white matter: 7.1 nTPM
  • thalamus: 6.8 nTPM
  • basal ganglia: 5.8 nTPM
  • hypothalamus: 5.6 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RFC4.

Disease | AllUniProt

Conditions RFC4 is implicated in, by any mechanism.

  • Morimoto-Ryu-Malicdan neuromuscular syndrome (MRMNS) MIM:621010

Disease | GeneticClinVar

11 pathogenic / likely-pathogenic of 90 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Morimoto-Ryu-Malicdan neuromuscular syndrome

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.9
gnomAD pLI
0
gnomAD missense Z
-0.29
DepMap mean gene effect
-1.2
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 15% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RFC4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RFC4 as an antibody target. Whether an autoantibody or antibody against RFC4 could matter depends on whether native RFC4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RFC4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RFC4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RFC4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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