Seroatlas · Human Serome Atlas

MBD2

Methyl-CpG-binding domain protein 2

Also known as: MBD2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9UBB5
Gene
MBD2
Ensembl
ENSG00000134046
Chromosome
18
Canonical length
411 aa
Protein class
Cancer-related genes, Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Canonical amino-acid sequenceUniProt

411 residues, UniProt reviewed canonical sequence.

>Q9UBB5|MBD2
     1  MRAHPGGGRC CPEQEEGESA AGGSGAGGDS AIEQGGQGSA LAPSPVSGVR REGARGGGRG
    61  RGRWKQAGRG GGVCGRGRGR GRGRGRGRGR GRGRGRPPSG GSGLGGDGGG CGGGGSGGGG
   121  APRREPVPFP SGSAGPGPRG PRATESGKRM DCPALPPGWK KEEVIRKSGL SAGKSDVYYF
   181  SPSGKKFRSK PQLARYLGNT VDLSSFDFRT GKMMPSKLQK NKQRLRNDPL NQNKGKPDLN
   241  TTLPIRQTAS IFKQPVTKVT NHPSNKVKSD PQRMNEQPRQ LFWEKRLQGL SASDVTEQII
   301  KTMELPKGLQ GVGPGSNDET LLSAVASALH TSSAPITGQV SAAVEKNPAV WLNTSQPLCK
   361  AFIVTDEDIR KQEERVQQVR KKLEEALMAD ILSRAADTEE MDIEMDSGDE A

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MBD2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.58
Highest tissue expression
33 nTPM

Expression across tissuesHPA

Tissue

  • tonsil: 33 nTPM
  • lymph node: 30 nTPM
  • testis: 30 nTPM
  • thyroid gland: 29 nTPM
  • esophagus: 29 nTPM
  • adipose tissue: 25 nTPM

Single-cell type

  • ocular epithelial cells: 399 nCPM
  • neutrophils: 342 nCPM
  • kupffer cells: 328 nCPM
  • urothelial cells: 280 nCPM
  • endometrial glandular cells: 270 nCPM
  • sertoli cells: 252 nCPM

Immune cell

  • non-classical monocyte: 5 nTPM
  • intermediate monocyte: 2.6 nTPM
  • T-reg: 1.5 nTPM
  • classical monocyte: 1.4 nTPM
  • naive B-cell: 1.3 nTPM
  • neutrophil: 1.2 nTPM

Brain region

  • cerebellum: 17 nTPM
  • hypothalamus: 16 nTPM
  • basal ganglia: 16 nTPM
  • cerebral cortex: 15 nTPM
  • midbrain: 15 nTPM
  • white matter: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.52
gnomAD pLI
0.35
gnomAD missense Z
2.11
DepMap mean gene effect
-0.09
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MBD2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MBD2 as an antibody target. Whether an autoantibody or antibody against MBD2 could matter depends on whether native MBD2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MBD2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MBD2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MBD2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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