Seroatlas · Human Serome Atlas

MAGI3

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3

Also known as: MAGI-3, MAGI3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q5TCQ9
Gene
MAGI3
Ensembl
ENSG00000081026
Chromosome
1
Canonical length
1481 aa
Protein class
Predicted intracellular proteins
Subcellular location
Cell Junctions

OverviewNCBI Gene

Predicted to enable frizzled binding activity. Predicted to be involved in signal transduction. Predicted to act upstream of or within positive regulation of JUN kinase activity. Located in cell junction. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

1481 residues, UniProt reviewed canonical sequence.

>Q5TCQ9|MAGI3
     1  MSKTLKKKKH WLSKVQECAV SWAGPPGDFG AEIRGGAERG EFPYLGRLRE EPGGGTCCVV
    61  SGKAPSPGDV LLEVNGTPVS GLTNRDTLAV IRHFREPIRL KTVKPGKVIN KDLRHYLSLQ
   121  FQKGSIDHKL QQVIRDNLYL RTIPCTTRAP RDGEVPGVDY NFISVEQFKA LEESGALLES
   181  GTYDGNFYGT PKPPAEPSPF QPDPVDQVLF DNEFDAESQR KRTTSVSKME RMDSSLPEEE
   241  EDEDKEAING SGNAENRERH SESSDWMKTV PSYNQTNSSM DFRNYMMRDE TLEPLPKNWE
   301  MAYTDTGMIY FIDHNTKTTT WLDPRLCKKA KAPEDCEDGE LPYGWEKIED PQYGTYYVDH
   361  LNQKTQFENP VEEAKRKKQL GQVEIGSSKP DMEKSHFTRD PSQLKGVLVR ASLKKSTMGF
   421  GFTIIGGDRP DEFLQVKNVL KDGPAAQDGK IAPGDVIVDI NGNCVLGHTH ADVVQMFQLV
   481  PVNQYVNLTL CRGYPLPDDS EDPVVDIVAA TPVINGQSLT KGETCMNPQD FKPGAMVLEQ
   541  NGKSGHTLTG DGLNGPSDAS EQRVSMASSG SSQPELVTIP LIKGPKGFGF AIADSPTGQK
   601  VKMILDSQWC QGLQKGDIIK EIYHQNVQNL THLQVVEVLK QFPVGADVPL LILRGGPPSP
   661  TKTAKMKTDK KENAGSLEAI NEPIPQPMPF PPSIIRSGSP KLDPSEVYLK SKTLYEDKPP
   721  NTKDLDVFLR KQESGFGFRV LGGDGPDQSI YIGAIIPLGA AEKDGRLRAA DELMCIDGIP
   781  VKGKSHKQVL DLMTTAARNG HVLLTVRRKI FYGEKQPEDD SSQAFISTQN GSPRLNRAEV
   841  PARPAPQEPY DVVLQRKENE GFGFVILTSK NKPPPGVIPH KIGRVIEGSP ADRCGKLKVG
   901  DHISAVNGQS IVELSHDNIV QLIKDAGVTV TLTVIAEEEH HGPPSGTNSA RQSPALQHRP
   961  MGQSQANHIP GDRSALEGEI GKDVSTSYRH SWSDHKHLAQ PDTAVISVVG SRHNQNLGCY
  1021  PVELERGPRG FGFSLRGGKE YNMGLFILRL AEDGPAIKDG RIHVGDQIVE INGEPTQGIT
  1081  HTRAIELIQA GGNKVLLLLR PGTGLIPDHG DWDINNPSSS NVIYDEQSPL PPSSHFASIF
  1141  EESHVPVIEE SLRVQICEKA EELKDIVPEK KSTLNENQPE IKHQSLLQKN VSKRDPPSSH
  1201  GHSNKKNLLK VENGVTRRGR SVSPKKPASQ HSEEHLDKIP SPLKNNPKRR PRDQSLSPSK
  1261  GENKSCQVST RAGSGQDQCR KSRGRSASPK KQQKIEGSKA PSNAEAKLLE GKSRRIAGYT
  1321  GSNAEQIPDG KEKSDVIRKD AKQNQLEKSR TRSPEKKIKR MVEKSLPSKM TNKTTSKEVS
  1381  ENEKGKKVTT GETSSSNDKI GENVQLSEKR LKQEPEEKVV SNKTEDHKGK ELEAADKNKE
  1441  TGRFKPESSS PVKKTLITPG PWKVPSGNKV TGTIGMAEKR Q

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAGI3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.49
Highest tissue expression
32 nTPM

Expression across tissuesHPA

Tissue

  • retina: 32 nTPM
  • lung: 13 nTPM
  • stomach: 11 nTPM
  • thyroid gland: 10 nTPM
  • pancreas: 10 nTPM
  • colon: 8.6 nTPM

Single-cell type

  • alveolar cells type 1: 1,288 nCPM
  • transitional alveolar cells: 1,218 nCPM
  • renal collecting duct intercalated cells: 1,060 nCPM
  • retinal amacrine cells: 665 nCPM
  • salivary ionocytes: 658 nCPM
  • retinal bipolar cells: 601 nCPM

Immune cell

  • naive CD4 T-cell: 0.2 nTPM
  • basophil: 0.1 nTPM
  • memory CD4 T-cell: 0.1 nTPM
  • myeloid DC: 0.1 nTPM
  • neutrophil: 0.1 nTPM
  • NK-cell: 0.1 nTPM

Brain region

  • cerebral cortex: 33 nTPM
  • midbrain: 31 nTPM
  • pons: 29 nTPM
  • basal ganglia: 27 nTPM
  • hypothalamus: 27 nTPM
  • white matter: 27 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.39
gnomAD pLI
0.03
gnomAD missense Z
2.33
DepMap mean gene effect
-0.06
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MAGI3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MAGI3 as an antibody target. Whether an autoantibody or antibody against MAGI3 could matter depends on whether native MAGI3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MAGI3 is annotated at the cell surface, where native MAGI3 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label MAGI3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MAGI3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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