Seroatlas · Human Serome Atlas

DHX34

Probable ATP-dependent RNA helicase DHX34

Also known as: DDX34, DHX34_HUMAN, KIAA0134

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q14147
Gene: DHX34
Ensembl: ENSG00000134815
Chromosome: 19
Canonical length: 1143 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. It is mapped to the glioma 19q tumor suppressor region and is a tumor suppressor candidate gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1143 residues, UniProt reviewed canonical sequence.

>Q14147|DHX34
     1  MPPPRTREGR DRRDHHRAPS EEEALEKWDW NCPETRRLLE DAFFREEDYI RQGSEECQKF
    61  WTFFERLQRF QNLKTSRKEE KDPGQPKHSI PALADLPRTY DPRYRINLSV LGPATRGSQG
   121  LGRHLPAERV AEFRRALLHY LDFGQKQAFG RLAKLQRERA ALPIAQYGNR ILQTLKEHQV
   181  VVVAGDTGCG KSTQVPQYLL AAGFSHVACT QPRRIACISL AKRVGFESLS QYGSQVGYQI
   241  RFESTRSAAT KIVFLTVGLL LRQIQREPSL PQYEVLIVDE VHERHLHNDF LLGVLQRLLP
   301  TRPDLKVILM SATINISLFS SYFSNAPVVQ VPGRLFPITV VYQPQEAEPT TSKSEKLDPR
   361  PFLRVLESID HKYPPEERGD LLVFLSGMAE ISAVLEAAQT YASHTQRWVV LPLHSALSVA
   421  DQDKVFDVAP PGVRKCILST NIAETSVTID GIRFVVDSGK VKEMSYDPQA KLQRLQEFWI
   481  SQASAEQRKG RAGRTGPGVC FRLYAESDYD AFAPYPVPEI RRVALDSLVL QMKSMSVGDP
   541  RTFPFIEPPP PASLETAILY LRDQGALDSS EALTPIGSLL AQLPVDVVIG KMLILGSMFS
   601  LVEPVLTIAA ALSVQSPFTR SAQSSPECAA ARRPLESDQG DPFTLFNVFN AWVQVKSERS
   661  RNSRKWCRRR GIEEHRLYEM ANLRRQFKEL LEDHGLLAGA QAAQVGDSYS RLQQRRERRA
   721  LHQLKRQHEE GAGRRRKVLR LQEEQDGGSS DEDRAGPAPP GASDGVDIQD VKFKLRHDLA
   781  QLQAAASSAQ DLSREQLALL KLVLGRGLYP QLAVPDAFNS SRKDSDQIFH TQAKQGAVLH
   841  PTCVFAGSPE VLHAQELEAS NCDGSRDDKD KMSSKHQLLS FVSLLETNKP YLVNCVRIPA
   901  LQSLLLFSRS LDTNGDCSRL VADGWLELQL ADSESAIRLL AASLRLRARW ESALDRQLAH
   961  QAQQQLEEEE EDTPVSPKEV ATLSKELLQF TASKIPYSLR RLTGLEVQNM YVGPQTIPAT
  1021  PHLPGLFGSS TLSPHPTKGG YAVTDFLTYN CLTNDTDLYS DCLRTFWTCP HCGLHAPLTP
  1081  LERIAHENTC PQAPQDGPPG AEEAALETLQ KTSVLQRPYH CEACGKDFLF TPTEVLRHRK
  1141  QHV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DHX34 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.3
Highest tissue expression: 21 nTPM

Expression across tissuesHPA

Tissue

testis: 21 nTPM
spleen: 12 nTPM
bone marrow: 11 nTPM
ovary: 10 nTPM
liver: 9.7 nTPM
cerebellum: 9.2 nTPM

Single-cell type

neutrophils: 423 nCPM
monocytes: 114 nCPM
late spermatids: 107 nCPM
early primary spermatocytes: 93 nCPM
neutrophil progenitors: 53 nCPM
macrophages: 48 nCPM

Immune cell

neutrophil: 12 nTPM
classical monocyte: 1.8 nTPM
intermediate monocyte: 1.8 nTPM
myeloid DC: 1.7 nTPM
memory CD8 T-cell: 1.5 nTPM
non-classical monocyte: 1.5 nTPM

Brain region

cerebral cortex: 21 nTPM
white matter: 15 nTPM
medulla oblongata: 13 nTPM
cerebellum: 13 nTPM
thalamus: 12 nTPM
basal ganglia: 12 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DHX34.

Disease | GeneticClinVar

3 pathogenic / likely-pathogenic of 335 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.77
gnomAD pLI: 0
gnomAD missense Z: -0.08
DepMap mean gene effect: 0.1
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DHX34 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DHX34 as an antibody target. Whether an autoantibody or antibody against DHX34 could matter depends on whether native DHX34 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DHX34 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DHX34 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...