Seroatlas · Human Serome Atlas

DAPK1

Death-associated protein kinase 1

Also known as: DAPK, DAPK1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P53355
Gene: DAPK1
Ensembl: ENSG00000196730
Chromosome: 9
Canonical length: 1430 aa
Protein class: Cancer-related genes, Enzymes, Human disease related genes, Predicted membrane proteins
Subcellular location: Centrosome

OverviewNCBI Gene

Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Canonical amino-acid sequenceUniProt

1430 residues, UniProt reviewed canonical sequence.

>P53355|DAPK1
     1  MTVFRQENVD DYYDTGEELG SGQFAVVKKC REKSTGLQYA AKFIKKRRTK SSRRGVSRED
    61  IEREVSILKE IQHPNVITLH EVYENKTDVI LILELVAGGE LFDFLAEKES LTEEEATEFL
   121  KQILNGVYYL HSLQIAHFDL KPENIMLLDR NVPKPRIKII DFGLAHKIDF GNEFKNIFGT
   181  PEFVAPEIVN YEPLGLEADM WSIGVITYIL LSGASPFLGD TKQETLANVS AVNYEFEDEY
   241  FSNTSALAKD FIRRLLVKDP KKRMTIQDSL QHPWIKPKDT QQALSRKASA VNMEKFKKFA
   301  ARKKWKQSVR LISLCQRLSR SFLSRSNMSV ARSDDTLDEE DSFVMKAIIH AINDDNVPGL
   361  QHLLGSLSNY DVNQPNKHGT PPLLIAAGCG NIQILQLLIK RGSRIDVQDK GGSNAVYWAA
   421  RHGHVDTLKF LSENKCPLDV KDKSGEMALH VAARYGHADV AQLLCSFGSN PNIQDKEEET
   481  PLHCAAWHGY YSVAKALCEA GCNVNIKNRE GETPLLTASA RGYHDIVECL AEHGADLNAC
   541  DKDGHIALHL AVRRCQMEVI KTLLSQGCFV DYQDRHGNTP LHVACKDGNM PIVVALCEAN
   601  CNLDISNKYG RTPLHLAANN GILDVVRYLC LMGASVEALT TDGKTAEDLA RSEQHEHVAG
   661  LLARLRKDTH RGLFIQQLRP TQNLQPRIKL KLFGHSGSGK TTLVESLKCG LLRSFFRRRR
   721  PRLSSTNSSR FPPSPLASKP TVSVSINNLY PGCENVSVRS RSMMFEPGLT KGMLEVFVAP
   781  THHPHCSADD QSTKAIDIQN AYLNGVGDFS VWEFSGNPVY FCCYDYFAAN DPTSIHVVVF
   841  SLEEPYEIQL NQVIFWLSFL KSLVPVEEPI AFGGKLKNPL QVVLVATHAD IMNVPRPAGG
   901  EFGYDKDTSL LKEIRNRFGN DLHISNKLFV LDAGASGSKD MKVLRNHLQE IRSQIVSVCP
   961  PMTHLCEKII STLPSWRKLN GPNQLMSLQQ FVYDVQDQLN PLASEEDLRR IAQQLHSTGE
  1021  INIMQSETVQ DVLLLDPRWL CTNVLGKLLS VETPRALHHY RGRYTVEDIQ RLVPDSDVEE
  1081  LLQILDAMDI CARDLSSGTM VDVPALIKTD NLHRSWADEE DEVMVYGGVR IVPVEHLTPF
  1141  PCGIFHKVQV NLCRWIHQQS TEGDADIRLW VNGCKLANRG AELLVLLVNH GQGIEVQVRG
  1201  LETEKIKCCL LLDSVCSTIE NVMATTLPGL LTVKHYLSPQ QLREHHEPVM IYQPRDFFRA
  1261  QTLKETSLTN TMGGYKESFS SIMCFGCHDV YSQASLGMDI HASDLNLLTR RKLSRLLDPP
  1321  DPLGKDWCLL AMNLGLPDLV AKYNTSNGAP KDFLPSPLHA LLREWTTYPE STVGTLMSKL
  1381  RELGRRDAAD FLLKASSVFK INLDGNGQEA YASSCNSGTS YNSISSVVSR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DAPK1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.25
Highest tissue expression: 46 nTPM

Expression across tissuesHPA

Tissue

ovary: 46 nTPM
thymus: 39 nTPM
lung: 35 nTPM
pancreas: 28 nTPM
gallbladder: 26 nTPM
liver: 26 nTPM

Single-cell type

mesothelial cells: 810 nCPM
epicardial cells: 707 nCPM
hematopoietic stem cells: 615 nCPM
renal collecting duct principal cells: 551 nCPM
transitional alveolar cells: 533 nCPM
thyrotrophs: 492 nCPM

Immune cell

non-classical monocyte: 15 nTPM
intermediate monocyte: 14 nTPM
eosinophil: 8.7 nTPM
classical monocyte: 6.7 nTPM
plasmacytoid DC: 6.2 nTPM
myeloid DC: 4.8 nTPM

Brain region

hippocampal formation: 55 nTPM
cerebral cortex: 50 nTPM
basal ganglia: 42 nTPM
pons: 37 nTPM
choroid plexus: 34 nTPM
amygdala: 32 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.28
gnomAD pLI: 1
gnomAD missense Z: 2.57
DepMap mean gene effect: 0.01
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

apoptotic process
apoptotic signaling pathway
cellular response to hydroperoxide
cellular response to type II interferon
defense response to tumor cell
extrinsic apoptotic signaling pathway via death domain receptors
intracellular signal transduction
negative regulation of apoptotic process
negative regulation of translation
positive regulation of apoptotic process
positive regulation of autophagy
protein autophosphorylation
protein phosphorylation
regulation of apoptotic process
regulation of autophagy
regulation of NMDA receptor activity
positive regulation of autophagic cell death
regulation of response to tumor cell

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DAPK1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DAPK1 as an antibody target. Whether an autoantibody or antibody against DAPK1 could matter depends on whether native DAPK1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DAPK1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DAPK1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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